Dietrich Mariola A, Nynca Joanna, Adamek Mikołaj, Steinhagen Dieter, Karol Halina, Ciereszko Andrzej
Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn, Department of Gamete and Embryo Biology, Poland.
Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn, Department of Gamete and Embryo Biology, Poland.
Fish Shellfish Immunol. 2015 Aug;45(2):750-6. doi: 10.1016/j.fsi.2015.05.048. Epub 2015 Jun 2.
Antimicrobial proteins such as apolipoproteins A (ApoA-I and ApoA-II) play an important role in the primary defence barrier in vertebrates including fish. The aims of the present study were to isolate and characterise rainbow trout seminal plasma ApoA-I and ApoA-II, to examine the mRNA expression of each apolipoprotein in testis and spermatic ducts, and to test the antibacterial properties of the apolipoproteins. Using a three-step isolation procedure consisting of ion-exchange chromatography, gel filtration and preparative SDS-PAGE, apolipoproteins were purified and identified as ApoA-I and ApoA-II. Both apolipoproteins were represented by several proteoforms. The expression of ApoA-I and ApoA-II mRNA in the reproductive tract and their antibacterial properties against Escherichia coli suggest that seminal apolipoproteins play an important role in innate immunity in the rainbow trout reproductive tract. The functions of seminal ApoA can be related to protection of sperm and reproductive tissue from microbial attack and to the maintenance of sperm membrane integrity.
抗菌蛋白如载脂蛋白A（载脂蛋白A-I和载脂蛋白A-II）在包括鱼类在内的脊椎动物的初级防御屏障中发挥着重要作用。本研究的目的是分离和鉴定虹鳟精浆中的载脂蛋白A-I和载脂蛋白A-II，检测睾丸和输精管中各载脂蛋白的mRNA表达，并测试载脂蛋白的抗菌特性。采用离子交换色谱、凝胶过滤和制备型SDS-PAGE三步分离法，对载脂蛋白进行了纯化和鉴定，确定为载脂蛋白A-I和载脂蛋白A-II。两种载脂蛋白均由多种蛋白形式组成。载脂蛋白A-I和载脂蛋白A-II mRNA在生殖道中的表达及其对大肠杆菌的抗菌特性表明，精浆载脂蛋白在虹鳟生殖道的固有免疫中起重要作用。精浆载脂蛋白A的功能可能与保护精子和生殖组织免受微生物攻击以及维持精子膜完整性有关。
