Koster Remco A, Botma Rixt, Greijdanus Ben, Uges Donald R A, Kosterink Jos G W, Touw Daan J, Alffenaar Jan-Willem C
Bioanalysis. 2015;7(10):1225-35. doi: 10.4155/bio.15.63.
The relation between hematocrit, substance concentration, extraction recovery and spot formation of tacrolimus, sirolimus, everolimus, ascomycin, temsirolimus and cyclosporin A was investigated for Whatman 31 ET CHR, Whatman FTA DMPK-C, Whatman 903, Perkin Elmer 226 and Agilent Bond Elut DMS DBS cards.
RESULTS & DISCUSSION: We found that all DBS cards showed the same hematocrit and concentration-dependent recovery patterns for sirolimus, everolimus and temsirolimus. At high concentrations, the total hematocrit effects were much more pronounced than at low concentrations for tacrolimus, sirolimus, everolimus, ascomycin and temsirolimus.
The tested card types showed differences in performance, especially at extreme concentrations and hematocrit values. It may be useful to investigate the performance of different types of DBS cards prior to analytical method validation.
针对Whatman 31 ET CHR、Whatman FTA DMPK-C、Whatman 903、珀金埃尔默226和安捷伦Bond Elut DMS DBS卡，研究了血细胞比容、物质浓度、他克莫司、西罗莫司、依维莫司、子囊霉素、替西罗莫司和环孢素A的提取回收率与斑点形成之间的关系。
我们发现，所有DBS卡对西罗莫司、依维莫司和替西罗莫司均呈现相同的血细胞比容和浓度依赖性回收率模式。在高浓度下，他克莫司、西罗莫司、依维莫司、子囊霉素和替西罗莫司的总血细胞比容效应比低浓度时更为明显。
所测试的卡类型在性能上存在差异，尤其是在极端浓度和血细胞比容值时。在分析方法验证之前研究不同类型DBS卡的性能可能会有所帮助。
