Hempen Christel Maria, Maarten Koster Emile Hermannus, Ooms Jan Albert
Spark Holland B.V., Pieter de Keyserstraat 8, 7825 VE Emmen, The Netherlands.
Bioanalysis. 2015;7(16):2019-29. doi: 10.4155/bio.15.97.
We applied our concept for automated flow-through desorption of DBS to investigate the effect of desorption temperature on recovery. For this purpose, a method has been developed for the determination of four immunosuppressants in DBS.
We compared recoveries of four immunosuppressants for measurements with and without temperature-enhanced desorption at different hematocrit (Ht) levels. Temperature-enhanced desorption increased recovery substantially for tacrolimus, sirolimus and everolimus at all Ht values and for cyclosporine At high Ht. In addition, recovery became largely independent from Ht variations. Under the optimized conditions, a brief validation using spiked blood samples showed that the method complies with acceptance criteria for quantitative bioanalysis.
This method enables a quantitative analysis of immunosuppressants in DBS independent from the Ht.
我们应用了干血斑自动流通解吸的概念来研究解吸温度对回收率的影响。为此，已开发出一种测定干血斑中四种免疫抑制剂的方法。
我们比较了在不同血细胞比容（Ht）水平下，有温度增强解吸和无温度增强解吸时四种免疫抑制剂的回收率。对于他克莫司、西罗莫司和依维莫司，在所有Ht值下，温度增强解吸均显著提高了回收率；对于环孢素，在高Ht时也是如此。此外，回收率在很大程度上不受Ht变化的影响。在优化条件下，使用加标血样进行的简短验证表明该方法符合定量生物分析的验收标准。
该方法能够独立于Ht对干血斑中的免疫抑制剂进行定量分析。
