趋化因子的达菲抗原受体在瘢痕疙瘩中的作用。

The role of Duffy antigen receptor for chemokines in keloids.

作者信息

Chen Ying, Liao Nong, Lu Feng, Peng Hui, Gao Jianhua

机构信息

Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China; Department of Breast Surgery, Guangdong Traditional Chinese Medicine Hospital, Guangzhou 510000, Guangdong, China.

Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China; Department of Plastic Surgery, The Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, Guangdong, China.

出版信息

Gene. 2015 Oct 1;570(1):44-9. doi: 10.1016/j.gene.2015.05.071. Epub 2015 Jun 2.

DOI:10.1016/j.gene.2015.05.071

PMID:26045366

Abstract

This study aims to determine the relationship between Duffy antigen receptor for chemokines (DARC) and keloid pathogenesis. DARC expression was determined by immunohistochemistry, real-time PCR, and Western blot analysis. Cell proliferation was assessed by CCK-8 assay. Cell migration and invasion abilities were measured by the shift assay. Levels of CC chemokine ligand 2 (CCL2), CXC chemokine ligand 8 (CXCL8), and matrix metalloproteinase 2 (MMP2) were detected by real-time PCR and ELISA. Our results showed that DARC levels were elevated in human keloid fibroblasts. After knocking down DARC, cell proliferation was not altered, whereas the migration and invasion abilities of keloid fibroblasts were significantly elevated. Additionally, the mRNA expression levels of CCL2, CXCL8, and MMP2 were not influenced by DARC knockdown. However, the secretion of CCL2, but not CXCL8 or MMP2, was significantly increased after DARC knockdown. Our results suggest that DARC might inhibit the secretion of CCL2. Moreover, DARC knockdown increases the migration and invasion abilities of keloid fibroblasts.

摘要

本研究旨在确定趋化因子的达菲抗原受体（DARC）与瘢痕疙瘩发病机制之间的关系。通过免疫组织化学、实时定量聚合酶链反应（PCR）和蛋白质免疫印迹分析来确定DARC的表达。采用细胞计数试剂盒-8（CCK-8）法评估细胞增殖。通过迁移实验测定细胞迁移和侵袭能力。采用实时定量PCR和酶联免疫吸附测定（ELISA）检测CC趋化因子配体2（CCL2）、CXC趋化因子配体8（CXCL8）和基质金属蛋白酶2（MMP2）的水平。我们的结果表明，人瘢痕疙瘩成纤维细胞中DARC水平升高。敲低DARC后，细胞增殖未发生改变，而瘢痕疙瘩成纤维细胞的迁移和侵袭能力显著提高。此外，CCL2、CXCL8和MMP2的信使核糖核酸（mRNA）表达水平不受DARC敲低的影响。然而，敲低DARC后，CCL2的分泌显著增加，而CXCL8或MMP2的分泌则未增加。我们的结果表明，DARC可能抑制CCL2的分泌。此外，敲低DARC可增加瘢痕疙瘩成纤维细胞的迁移和侵袭能力。