Mou Wangshu, Li Dongdong, Luo Zisheng, Mao Linchun, Ying Tiejin
College of Biosystems Engineering and Food Science, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang R & D Center for Food Technology and Equipment, Zhejiang University, Hangzhou, People's Republic of China.
PLoS One. 2015 Jun 8;10(6):e0129598. doi: 10.1371/journal.pone.0129598. eCollection 2015.
Abscisic acid (ABA) has been proven to be involved in the regulation of climacteric fruit ripening, but a comprehensive investigation of its influence on ripening related processes is still lacking. By applying the next generation sequencing technology, we conducted a comparative analysis of the effects of exogenous ABA and NDGA (Nordihydroguaiaretic acid, an inhibitor of ABA biosynthesis) on tomato fruit ripening. The high throughput sequencing results showed that out of the 25728 genes expressed across all three samples, 10388 were identified as significantly differently expressed genes. Exogenous ABA was found to enhance the transcription of genes involved in pigments metabolism, including carotenoids biosynthesis and chlorophyll degradation, whereas NDGA treatment inhibited these processes. The results also revealed the crucial role of ABA in flavonoids synthesis and regulation of antioxidant system. Intriguingly, we also found that an inhibition of endogenous ABA significantly enhanced the transcriptional abundance of genes involved in photosynthesis. Our results highlighted the significance of ABA in regulating tomato ripening, which provided insight into the regulatory mechanism of fruit maturation and senescence process.
脱落酸（ABA）已被证明参与呼吸跃变型果实的成熟调控，但目前仍缺乏对其对成熟相关过程影响的全面研究。通过应用新一代测序技术，我们对外源ABA和NDGA（去甲二氢愈创木酸，一种ABA生物合成抑制剂）对番茄果实成熟的影响进行了比较分析。高通量测序结果显示，在所有三个样本中表达的25728个基因中，有10388个被鉴定为显著差异表达基因。研究发现，外源ABA可增强参与色素代谢的基因的转录，包括类胡萝卜素生物合成和叶绿素降解，而NDGA处理则抑制了这些过程。结果还揭示了ABA在黄酮类化合物合成和抗氧化系统调节中的关键作用。有趣的是，我们还发现抑制内源性ABA可显著增强参与光合作用的基因的转录丰度。我们的结果突出了ABA在调控番茄成熟中的重要性，这为果实成熟和衰老过程的调控机制提供了见解。
