在小鼠卵母细胞减数分裂成熟过程中，染色体分离需要Nuf2。
Nuf2 is required for chromosome segregation during mouse oocyte meiotic maturation.
作者信息
Zhang Teng, Zhou Yang, Qi Shu-Tao, Wang Zhen-Bo, Qian Wei-Ping, Ouyang Ying-Chun, Shen Wei, Schatten Heide, Sun Qing-Yuan
机构信息
a Institute of Reproductive Sciences; College of Animal Science and Technology; Qingdao Agricultural University ; Qingdao , China.
出版信息
Cell Cycle. 2015;14(16):2701-10. doi: 10.1080/15384101.2015.1058677.
Abstract
Nuf2 plays an important role in kinetochore-microtubule attachment and thus is involved in regulation of the spindle assembly checkpoint in mitosis. In this study, we examined the localization and function of Nuf2 during mouse oocyte meiotic maturation. Myc6-Nuf2 mRNA injection and immunofluorescent staining showed that Nuf2 localized to kinetochores from germinal vesicle breakdown to metaphase I stages, while it disappeared from the kinetochores at the anaphase I stage, but relocated to kinetochores at the MII stage. Overexpression of Nuf2 caused defective spindles, misaligned chromosomes, and activated spindle assembly checkpoint, and thus inhibited chromosome segregation and metaphase-anaphase transition in oocyte meiosis. Conversely, precocious polar body extrusion was observed in the presence of misaligned chromosomes and abnormal spindle formation in Nuf2 knock-down oocytes, causing aneuploidy. Our data suggest that Nuf2 is a critical regulator of meiotic cell cycle progression in mammalian oocytes.
摘要
Nuf2在动粒-微管附着中起重要作用，因此参与有丝分裂中纺锤体组装检查点的调控。在本研究中，我们检测了Nuf2在小鼠卵母细胞减数分裂成熟过程中的定位和功能。Myc6-Nuf2 mRNA注射和免疫荧光染色显示，从生发泡破裂到中期I阶段，Nuf2定位于动粒，而在后期I阶段它从动粒消失，但在MII阶段又重新定位于动粒。Nuf2的过表达导致纺锤体缺陷、染色体排列错误并激活纺锤体组装检查点，从而抑制卵母细胞减数分裂中的染色体分离和中期-后期转换。相反，在Nuf2敲低的卵母细胞中，观察到染色体排列错误和纺锤体形成异常时会出现早熟的极体排出，导致非整倍体。我们的数据表明，Nuf2是哺乳动物卵母细胞减数分裂细胞周期进程的关键调节因子。
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