Key Laboratory for Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, PR China.
Key Laboratory for Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, PR China.
Sci Total Environ. 2015 Nov 1;532:31-9. doi: 10.1016/j.scitotenv.2015.06.002. Epub 2015 Jun 6.
The aim of this study was to evaluate the trophic transfer of the β-blocker propranolol (PRP) in an experimental aquatic food chain involving the green algae Scenedesmus obliquus, the water flea Daphnia magna and the crucian carp Carassius auratus, as well as the metabolism and effects of PRP in the liver of crucian carp. After a 48 h PRP aqueous exposure for algae, with a subsequent 48 h dietary exposure for daphnia and an 8d dietary exposure for crucian carp, PRP was observed in each trophic level, despite significant bioaccumulation did not occur in daphnia and crucian carp. A portion of the absorbed PRP was metabolized by the crucian carp to N-desisopropylated propranolol, propranolol glucuronic acid, monohydroxylated propranolol, hydroxypropranolol glucuronide and dihydroxypropranolol glucuronide, which were similar to those in mammals. In addition, multiple biomarkers in the liver of crucian carp (7-ethoxyresorufin O-deethylase, EROD; 7-benzyloxyresorufin O-dealkylation, BROD; superoxide dismutase, SOD and malondialdehyde, MDA) were measured. BROD and MDA were not significantly affected by PRP, while EROD and SOD did change significantly during the 8d dietary exposure. This work indicated that the trophic transfer of PRP, resulting in biochemical perturbations of fish biological systems, should be a concern for the assessment of the environmental risks to aquatic food chains.
本研究旨在评估β受体阻滞剂普萘洛尔（PRP）在涉及绿藻斜生栅藻、水蚤大型蚤和鲫鱼的实验性水生食物链中的营养传递，以及 PRP 在鲫鱼肝脏中的代谢和作用。在藻类进行 48 h 的 PRP 水暴露后，随后对水蚤进行 48 h 的饮食暴露，对鲫鱼进行 8d 的饮食暴露，尽管在水蚤和鲫鱼中未发生明显的生物蓄积，但仍观察到 PRP 存在于每个营养级中。鲫鱼吸收的一部分 PRP 被代谢为 N-去异丙基普萘洛尔、普萘洛尔葡萄糖醛酸、单羟基普萘洛尔、羟脯氨酸葡萄糖醛酸和二羟基普萘洛尔葡萄糖醛酸，这与哺乳动物中的代谢产物相似。此外，还测量了鲫鱼肝脏中的多种生物标志物（7-乙氧基resorufin O-去乙基酶，EROD；7-苄氧基resorufin O-脱烷基化，BROD；超氧化物歧化酶，SOD 和丙二醛，MDA）。PRP 对 BROD 和 MDA 没有显著影响，而 EROD 和 SOD 在 8d 的饮食暴露期间确实发生了显著变化。这项工作表明，PRP 的营养传递导致鱼类生物系统的生化扰动，应该引起对水生食物链环境风险评估的关注。
