Zhang Min, Yin Daqiang, Kong Fanxiang
State Key laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, PR China.
Ecotoxicol Environ Saf. 2008 Oct;71(2):384-9. doi: 10.1016/j.ecoenv.2007.10.014. Epub 2007 Dec 11.
Pentachlorophenol (PCP), which was reported to be a typical persistent organic pollutant and environmental endocrine disruptor, may cause threat to aquatic species. In this study, serum testosterone concentration, activity of liver microsome ethoxyresorufin O-deethylase (EROD) and glutathione S-transferases (GST) of crucian carp (Carassius carassius) exposed to PCP for 7 and 15 d, respectively, were examined. The results showed that testosterone concentration was induced remarkably after 7 d (P<0.05), and the testosterone concentrations in 15 d treatment crucian carp were higher than those in 7 d treatment. It was found that there were significant effects on activities of EROD and GST after crucian carp were exposed to PCP for 7 and 15 d (P<0.05), compared to the controls. EROD and GST activities increased with increase in PCP concentration and also with increase in time on exposure. The results indicated that serum testosterone, EROD and GST were sensitive endpoints to PCP. PCP may have endocrine disrupting activities and may affect the reproductive success of this species. It is possible that the changes of hepatic microsome enzyme activities may result in alterations of serum testosterone levels in crucian carp.
五氯苯酚（PCP）据报道是一种典型的持久性有机污染物和环境内分泌干扰物，可能对水生物种造成威胁。在本研究中，分别检测了暴露于PCP 7天和15天的鲫鱼（Carassius carassius）的血清睾酮浓度、肝脏微粒体乙氧基异吩恶唑酮O-脱乙基酶（EROD）和谷胱甘肽S-转移酶（GST）的活性。结果表明，7天后睾酮浓度显著升高（P<0.05），且15天处理组鲫鱼的睾酮浓度高于7天处理组。研究发现，鲫鱼暴露于PCP 7天和15天后，EROD和GST的活性受到显著影响（P<0.05），与对照组相比。EROD和GST的活性随PCP浓度的增加以及暴露时间的延长而增加。结果表明，血清睾酮、EROD和GST是PCP的敏感指标。PCP可能具有内分泌干扰活性，并可能影响该物种的繁殖成功率。肝脏微粒体酶活性的变化可能导致鲫鱼血清睾酮水平的改变。
