College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
College of Agriculture and Biotechnology, China Agricultural University, Beijing, China; College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao, China.
Aquat Toxicol. 2014 Oct;155:84-90. doi: 10.1016/j.aquatox.2014.06.011. Epub 2014 Jun 24.
The occurrence and fate of endocrine disrupting chemicals (EDCs) in aquatic species have attracted close attention during the last decades. In this study, the bioaccumulation and biotransformation of synthetic estrogen quinestrol, one of the typical EDCs, in the plasma and liver of crucian carp, were investigated by a newly developed and validated reversed-phase high performance liquid chromatography with fluorescent detection method. Crucian carp were exposed to quinestrol in concentration of 2, 10, 50, 100 μg/L (5.49, 27.43, 137.17, 274.34 nmol/L) for 60 days. After 60 days' exposure, the concentrations of quinestrol found in liver and plasma were in the range of 0.25-0.69 mg/kg and 0.19-0.30 mg/L respectively, positively correlated with the exposure concentrations ranged 2-100 μg/L (5.49-274.34 nmol/L). There was a negative correlation between the bio-accumulation ratios and the exposure concentrations of quinestrol. 17α-Ethinylestradiol was also found in liver and plasma, and the concentrations were 0.02-0.19 mg/kg and 0.37-0.96 mg/L, respectively. The results indicated that quinestrol can be accumulated and transformed to 17α-ethinylestradiol in crucian carp. Moreover, exposure to quinestrol caused oxidative damages to crucian carp and the content of malondialdehyde increased in all treatment concentrations.
在过去的几十年中，内分泌干扰化学物质（EDCs）在水生生物中的存在和命运引起了密切关注。在这项研究中，通过新开发的反相高效液相色谱法荧光检测方法，研究了合成雌激素己烯雌酚（一种典型的 EDC）在鲫鱼血浆和肝脏中的生物积累和生物转化。鲫鱼暴露于浓度为 2、10、50、100μg/L（5.49、27.43、137.17、274.34nmol/L）的己烯雌酚中 60 天。暴露 60 天后，在肝和血浆中发现的己烯雌酚浓度分别为 0.25-0.69mg/kg 和 0.19-0.30mg/L，与 2-100μg/L（5.49-274.34nmol/L）的暴露浓度呈正相关。生物积累比与己烯雌酚的暴露浓度呈负相关。17α-乙炔雌二醇也在肝和血浆中被发现，浓度分别为 0.02-0.19mg/kg 和 0.37-0.96mg/L。结果表明，己烯雌酚可在鲫鱼中积累并转化为 17α-乙炔雌二醇。此外，己烯雌酚暴露导致鲫鱼氧化损伤，所有处理浓度的丙二醛含量增加。
