嗜热毛壳菌55家族β-1,3-葡聚糖酶的表达、纯化及结晶
Expression, purification and crystallization of a family 55 β-1,3-glucanase from Chaetomium thermophilum.
作者信息
Papageorgiou Anastassios C, Li Duochuan
机构信息
Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Tykistokatu 6, 20521 Turku, Finland.
Department of Environmental Biology, Shandong Agricultural University, Taian, Shandong 271018, People's Republic of China.
出版信息
Acta Crystallogr F Struct Biol Commun. 2015 Jun;71(Pt 6):680-3. doi: 10.1107/S2053230X15006366. Epub 2015 May 20.
Abstract
A β-1,3-glucanase from the thermophilic fungus Chaetomium thermophilum was overexpressed in Pichia pastoris, purified and crystallized in the presence of 1.8 M sodium/potassium phosphate pH 6.8 as a precipitant. Data to 2.0 Å resolution were collected in-house at 293 K from a single crystal. The crystal was found to belong to space group P2(1), with unit-cell parameters a = 64.1, b = 85.8, c = 68.5 Å, β = 93.1° and one molecule in the asymmetric unit.
摘要
嗜热毛壳菌的一种β-1,3-葡聚糖酶在毕赤酵母中过表达，经纯化后，以1.8 M磷酸钠/钾（pH 6.8）作为沉淀剂进行结晶。在293 K下，从一块单晶内部收集到了分辨率为2.0 Å的数据。该晶体属于空间群P2(1)，晶胞参数为a = 64.1、b = 85.8、c = 68.5 Å，β = 93.1°，不对称单元中有一个分子。
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