用克劳氏芽孢杆菌NM-1的β-1,3-葡聚糖酶从海带多糖制备的β-1,3-葡聚糖低聚物诱导人外周血单核细胞产生肿瘤坏死因子-α 。

Induction of TNF-alpha production from human peripheral blood monocytes with beta-1,3-glucan oligomer prepared from laminarin with beta-1,3-glucanase from Bacillus clausii NM-1.

作者信息

Miyanishi Nobumitsu, Iwamoto Yoshiko, Watanabe Etsuo, Odaz Tatsuya

机构信息

Department of Food Science and Technology, Tokyo University of Fisheries, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.

出版信息

J Biosci Bioeng. 2003;95(2):192-5. doi: 10.1016/s1389-1723(03)80128-7.

DOI:10.1016/s1389-1723(03)80128-7

PMID:16233391

Abstract

We prepared a beta-1,3-glucan oligomer (DP> or = 4) from laminarin (DP: 25-30) derived from Laminaria digitata with beta-1,3-glucanase, and examined its effect on human peripheral blood monocytes. Conditioned medium prepared by incubating monocytes (MC-CM) with the beta-1,3-glucan oligomer showed strong inhibitory activity against the proliferation of human leukemic U937 cells. Since the beta-1,3-glucan oligomer had no direct cytotoxic effect on U937 cells up to 1000 microg/ml, the cytotoxicity of the MC-CM may be due to cytotoxic cytokines produced from monocytes stimulated by the beta-1,3-glucan oligomer. On the other hand, the MC-CM prepared with original laminarin had little effect on the growth of U937 cells. The cytotoxicity of the MC-CM prepared with the beta-1,3-glucan oligomer was significantly reduced by an anti-TNF-alpha antibody, but the anti-TNF-beta antibody had no effect. Our results suggest that the enzymatically depolymerized beta-1,3-glucan oligomer induces TNF-alpha production from human monocytes.

摘要

我们用来源于掌状海带的海带多糖（聚合度：25 - 30），通过β-1,3-葡聚糖酶制备了一种β-1,3-葡聚糖低聚物（聚合度≥4），并检测了其对人外周血单核细胞的影响。用β-1,3-葡聚糖低聚物孵育单核细胞制备的条件培养基（MC-CM）对人白血病U937细胞的增殖表现出强烈的抑制活性。由于β-1,3-葡聚糖低聚物在浓度高达1000微克/毫升时对U937细胞没有直接的细胞毒性作用，MC-CM的细胞毒性可能归因于由β-1,3-葡聚糖低聚物刺激单核细胞产生的细胞毒性细胞因子。另一方面，用原始海带多糖制备的MC-CM对U937细胞的生长几乎没有影响。用抗TNF-α抗体可显著降低用β-1,3-葡聚糖低聚物制备的MC-CM的细胞毒性，但抗TNF-β抗体则没有作用。我们的结果表明，酶解解聚的β-1,3-葡聚糖低聚物可诱导人单核细胞产生TNF-α。