Yu Fei, Lv Mingli, Li Dan, Cai Haidong, Ma Lishui, Luo Qiong, Yuan Xueyu, Lv Zhongwei
Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China (mainland).
Med Sci Monit. 2015 Jun 10;21:1687-92. doi: 10.12659/MSM.893522.
This study aimed to investigate the relationship between miR-506 and proliferation and migration of breast cancer cells.
MiR-506 mimics, inhibitor, and negative control (NC) were transfected into MDA-MB-231 breast cancer cells. Cell proliferation, cell counting, colony formation assay, and Transwell assay were applied to evaluate the proliferation and migration of breast cancer cells. Data are shown as mean ± standard deviation and the experiment was performed 3 times. Statistical analyses were performed with SPSS version 10.0.
At 1 day after transfection, cell proliferation detected by CCK-8 assay was significantly promoted in miR-506 inhibitor when compared with the miR-506 mimics group and the NC group (P<0.05). At 3 days or 5 days after transfection, cell proliferation was markedly inhibited in the miR-506 mimics group, and miR-506 inhibitor was still significantly promoted. Cell counting with a hemocytometer showed similar results to cell proliferation. Colony formation assay showed that the number of colonies in the miR-506 mimics group was significantly smaller than that in the miR-506 inhibitor group and NC group. Transwell assay revealed that the number of migrated cells in miR-506 mimics was markedly smaller than that in the miR-506 inhibitor group and NC group.
MiR-506 over-expression significantly inhibits the proliferation, colony formation, and migration of breast cancer cells. miR-506 over-expression may thus be able to improve the malignant phenotype of breast cancer cells.
本研究旨在探讨miR-506与乳腺癌细胞增殖和迁移之间的关系。
将miR-506模拟物、抑制剂和阴性对照（NC）转染至MDA-MB-231乳腺癌细胞中。应用细胞增殖、细胞计数、集落形成试验和Transwell试验评估乳腺癌细胞的增殖和迁移。数据以平均值±标准差表示，实验重复进行3次。使用SPSS 10.0版进行统计分析。
转染后1天，与miR-506模拟物组和NC组相比，miR-506抑制剂组通过CCK-8试验检测的细胞增殖显著促进（P<0.05）。转染后3天或5天，miR-506模拟物组细胞增殖明显受到抑制，而miR-506抑制剂组仍显著促进。血细胞计数器细胞计数结果与细胞增殖结果相似。集落形成试验表明，miR-506模拟物组的集落数明显少于miR-506抑制剂组和NC组。Transwell试验显示，miR-506模拟物组迁移的细胞数明显少于miR-506抑制剂组和NC组。
miR-506过表达显著抑制乳腺癌细胞的增殖、集落形成和迁移。因此，miR-506过表达可能能够改善乳腺癌细胞的恶性表型。
