巨噬细胞摄取过程中单个粒子旋转的追踪
Tracking single-particle rotation during macrophage uptake.
作者信息
Sanchez Lucero, Patton Paul, Anthony Stephen M, Yi Yi, Yu Yan
机构信息
Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
出版信息
Soft Matter. 2015 Jul 14;11(26):5346-52. doi: 10.1039/c5sm00893j. Epub 2015 Jun 10.
Abstract
We investigated the rotational dynamics of single microparticles during their internalization by macrophage cells. The microparticles used were triblock patchy particles that display two fluorescent patches on their two poles. The optical anisotropy made it possible to directly visualize and quantify the orientation and rotation of the particles. We show that particles exhibit a mixture of fast and slow rotation as they are uptaken by macrophages and transiently undergo directional rotation during their entry into the cell. The size of the particles and the surface presentation of ligands exerted a negligible influence on this heterogeneity of particle rotation.
摘要
我们研究了单个微粒在被巨噬细胞内化过程中的旋转动力学。所使用的微粒是三嵌段斑点微粒，在其两极显示两个荧光斑点。光学各向异性使得直接可视化和量化微粒的取向和旋转成为可能。我们发现，微粒在被巨噬细胞摄取时呈现出快速和缓慢旋转的混合状态，并且在进入细胞的过程中会短暂地进行定向旋转。微粒的大小和配体的表面呈现对微粒旋转的这种异质性影响可忽略不计。
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