非洲爪蟾卵母细胞中形成的细胞内微小RNA图谱可能有助于不对称细胞分裂。

Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division.

作者信息

Sidova Monika, Sindelka Radek, Castoldi Mirco, Benes Vladimir, Kubista Mikael

机构信息

1] Laboratory of Gene Expression, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Videnska 1083, Prague, 142 20 Czech Republic [2] Charles University in Prague, Faculty of Science, Department of Cell Biology, Vinicna 7, Prague, 128 43, Czech Republic.

Laboratory of Gene Expression, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Videnska 1083, Prague, 142 20 Czech Republic.

出版信息

Sci Rep. 2015 Jun 10;5:11157. doi: 10.1038/srep11157.

DOI:10.1038/srep11157

PMID:26059897

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4461913/

Abstract

Asymmetric distribution of fate determinants within cells is an essential biological strategy to prepare them for asymmetric division. In this work we measure the intracellular distribution of 12 maternal microRNAs (miRNA) along the animal-vegetal axis of the Xenopus laevis oocyte using qPCR tomography. We find the miRNAs have distinct intracellular profiles that resemble two out of the three profiles we previously observed for mRNAs. Our results suggest that miRNAs in addition to proteins and mRNAs may have asymmetric distribution within the oocyte and may contribute to asymmetric cell division as cell fate determinants.

摘要

细胞内命运决定因子的不对称分布是使细胞为不对称分裂做准备的一种重要生物学策略。在这项研究中，我们使用定量PCR断层扫描技术测量了非洲爪蟾卵母细胞中12种母体微小RNA（miRNA）沿动物 - 植物轴的细胞内分布。我们发现这些miRNA具有独特的细胞内分布模式，类似于我们之前观察到的三种mRNA分布模式中的两种。我们的结果表明，除了蛋白质和mRNA之外，miRNA在卵母细胞内可能也具有不对称分布，并且可能作为细胞命运决定因子参与不对称细胞分裂。