Wellcome Trust/Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.
Nat Rev Mol Cell Biol. 2011 Jun 23;12(7):453-9. doi: 10.1038/nrm3140.
Differentiated cells can be experimentally reprogrammed back to pluripotency by nuclear transfer, cell fusion or induced pluripotent stem cell technology. Nuclear transfer and cell fusion can lead to efficient reprogramming of gene expression. The egg and oocyte reprogramming process includes the exchange of somatic proteins for oocyte proteins, the post-translational modification of histones and the demethylation of DNA. These events occur in an ordered manner and on a defined timescale, indicating that reprogramming by nuclear transfer and by cell fusion rely on deterministic processes.
分化细胞可通过核移植、细胞融合或诱导多能干细胞技术被实验性地重新编程为多能性。核移植和细胞融合可以导致基因表达的高效重编程。卵母细胞和卵子的重编程过程包括体细胞蛋白与卵母细胞蛋白的交换、组蛋白的翻译后修饰和 DNA 的去甲基化。这些事件以有序的方式和在确定的时间尺度上发生，表明核移植和细胞融合的重编程依赖于确定性过程。
