Gao Chengcheng, Ma Tianheng, Pang Liqun, Xie Rui
Department of Gastroenterology, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, P. R. China.
Afr Health Sci. 2014 Mar;14(1):206-10. doi: 10.4314/ahs.v14i1.32.
Herbs might affect the homeostasis of bile acids through influence of multiple metabolic pathways of bile acids.
To investigate the inhibition of cryptotanshinone towards the glucuronidation of LCA, trying to indicate the possible influence of cryptotanshinone-containing herbs towards the homeostasis of bile acids.
The LCA-3-glucuronidation and LCA-24-glucuronidation reaction was monitored by LC-MS.
Initial screening showed that 100 µM of cryptotanshinone inhibited LCA-24-glucuronidation and LCA-3-glucuronidation reaction activity by 82.6% and 79.1%, respectively. This kind of inhibition behaviour exerted cryptotanshinone concentrations-dependent and LCA concentrations-independent inhibition behaviour.
All these data indicated the possibility of cryptotanshinone's influence towards bile acids metabolism and homeostasis of bile acids.
草药可能通过影响胆汁酸的多种代谢途径来影响胆汁酸的稳态。
研究隐丹参酮对LCA葡萄糖醛酸化的抑制作用，试图阐明含隐丹参酮的草药对胆汁酸稳态可能产生的影响。
采用液相色谱-质谱联用技术监测LCA-3-葡萄糖醛酸化和LCA-24-葡萄糖醛酸化反应。
初步筛选表明，100 μM隐丹参酮分别抑制LCA-24-葡萄糖醛酸化和LCA-3-葡萄糖醛酸化反应活性82.6%和79.1%。这种抑制行为表现为隐丹参酮浓度依赖性和LCA浓度非依赖性抑制行为。
所有这些数据表明隐丹参酮影响胆汁酸代谢及胆汁酸稳态的可能性。
