Jiang Likun, Zhang Songhao, Chen Baohua, Mahboob Shahid, Al-Ghanim Khalid A, Feng Jianxin
a College of Life Sciences, Shanghai Ocean University , Shanghai , China .
b College of Life Sciences, Tianjin Normal University , Tianjin , China .
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2531-2. doi: 10.3109/19401736.2015.1038790. Epub 2015 Jun 10.
The complete mitochondrial genomes of two ornamental fishes, black molly (Poecilia sphenops) and blue gourami (Trichogaster trichopterus), were obtained by the traditional polymerase chain reaction (PCR)-based sequencing approach. The mitogenomes of P. sphenops and T. trichopterus are determined as 16,533 bp and 16,456 bp in length, respectively. Both the genomes include 22 transfer RNA genes, 13 protein-coding genes and 2 ribosomal RNA genes. Phylogenetic tree was constructed based on the complete mitogenomes of these two species and closely related 20 teleost species to assess their phylogenic relationship and evolution.
通过基于传统聚合酶链反应（PCR）的测序方法，获得了两种观赏鱼黑摩利（Poecilia sphenops）和蓝曼龙（Trichogaster trichopterus）的完整线粒体基因组。黑摩利和蓝曼龙的线粒体基因组长度分别确定为16,533 bp和16,456 bp。这两个基因组均包含22个转运RNA基因、13个蛋白质编码基因和2个核糖体RNA基因。基于这两个物种以及与之亲缘关系较近的20种硬骨鱼的完整线粒体基因组构建了系统发育树，以评估它们的系统发育关系和进化情况。
