Tang Wenjun, Xia Jianye, Chu Ju, Zhuang Yingping, Zhang Siliang
Sheng Wu Gong Cheng Xue Bao. 2015 Feb;31(2):291-9.
Filamentous fungi are widely used in industrial fermentation. Particular fungal morphology acts as a critical index for a successful fermentation. To break the bottleneck of morphological analysis, we have developed a reliable method for fungal morphological analysis. By this method, we can prepare hundreds of pellet samples simultaneously and obtain quantitative morphological information at large scale quickly. This method can largely increase the accuracy and reliability of morphological analysis result. Based on that, the studies of Aspergillus niger morphology under different oxygen supply conditions and shear rate conditions were carried out. As a result, the morphological responding patterns of A. niger morphology to these conditions were quantitatively demonstrated, which laid a solid foundation for the further scale-up.
丝状真菌在工业发酵中被广泛应用。特定的真菌形态是成功发酵的关键指标。为突破形态分析的瓶颈，我们开发了一种可靠的真菌形态分析方法。通过该方法，我们可以同时制备数百个菌球样本，并快速大规模获取定量形态信息。此方法能大幅提高形态分析结果的准确性和可靠性。在此基础上，开展了黑曲霉在不同供氧条件和剪切速率条件下的形态研究。结果定量地揭示了黑曲霉形态对这些条件的响应模式，为进一步扩大规模奠定了坚实基础。
