Mertens-Talcott Susanne U, Percival Susan S, Talcott Stephen T
Department of Nutrition and Food Science, Texas A&M University, Centeq A #220F, TAMU 2254, 1500 Research Parkway A, College Station, TX 77843-2254, United States.
Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL 32611, United States.
Food Chem. 2008 Jun 1;108(3):824-32. doi: 10.1016/j.foodchem.2007.11.037. Epub 2007 Nov 23.
Red wine contains a diversity of polyphenolic compounds that exert beneficial health effects including anti-cancer effects. This trial evaluated the anti-proliferative potential of red muscadine (Vitis rotundifolia) and red cabernet sauvignon (Vitis vinifera) wines in cell culture. Chemical properties of wines were determined by HPLC-PDA analysis and concentrated extracts of each wine were evaluated before and after glycosidic hydrolysis in MOLT-4 leukemia cells. Cell growth and the induction of apoptosis were evaluated after exposure to various extract dilutions. Wine extracts reduced cell viability up to 68% and cell numbers up to 50% after 48h with muscadine extracts being more effective than cabernet sauvignon. Caspase-3 activity was induced similarly by all extracts in a dose dependent manner. Cell cycle arrest in the G2/M phase was observed for both muscadine and the non-hydrolyzed cabernet sauvignon extract. Collectively, extracts from both wines exerted anti-cancer effects in leukemia cells.
红酒含有多种多酚化合物，这些化合物具有有益健康的作用，包括抗癌作用。本试验评估了红圆叶葡萄（Vitis rotundifolia）和赤霞珠红葡萄酒（Vitis vinifera）在细胞培养中的抗增殖潜力。通过高效液相色谱-光电二极管阵列（HPLC-PDA）分析测定葡萄酒的化学性质，并在MOLT-4白血病细胞中对每种葡萄酒的浓缩提取物进行糖苷水解前后的评估。在暴露于各种提取物稀释液后，评估细胞生长和凋亡诱导情况。48小时后，葡萄酒提取物可使细胞活力降低高达68%，细胞数量降低高达50%，其中圆叶葡萄提取物比赤霞珠更有效。所有提取物均以剂量依赖性方式类似地诱导半胱天冬酶-3活性。圆叶葡萄和未水解的赤霞珠提取物均观察到细胞周期停滞在G2/M期。总体而言，两种葡萄酒的提取物均对白血病细胞发挥抗癌作用。
