Angolini Célio Fernando F, Vendramini Pedro Henrique, Araújo Francisca D S, Araújo Welington L, Augusti Rodinei, Eberlin Marcos N, de Oliveira Luciana Gonzaga
†Department of Organic Chemistry, Institute of Chemistry, University of Campinas, UNICAMP, 13083-970 Campinas, SP, Brazil.
‡Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, USP, 05508-900 São Paulo, SP, Brazil.
Anal Chem. 2015 Jul 7;87(13):6925-30. doi: 10.1021/acs.analchem.5b01538. Epub 2015 Jun 25.
Herein we describe a new protocol that allows direct mass spectrometry imaging (IMS) of agar cultures. A simple sample dehydration leads to a thin solid agar, which enables the direct use of spray-based ambient mass spectrometry techniques. To demonstrate its applicability, metal scavengers siderophores were imaged directly from agar culture of S. wadayamensis, and well resolved and intense images were obtained using both desorption electrospray ionization (DESI) and easy ambient sonic-spray ionization (EASI) with well-defined selective spatial distributions for the free and the metal-bound molecules, providing clues for their roles in cellular metabolism.
