Girod D A, Duckert L G, Rubel E W
Department of Otolaryngology-Head and Neck Surgery, University of Washington, Seattle 98195.
Hear Res. 1989 Nov;42(2-3):175-94. doi: 10.1016/0378-5955(89)90143-3.
Hair cell regeneration following acoustic trauma to the avian cochlea has been documented using DNA labeling with tritiated thymidine. The goal of this study was to identify potential precursor cell populations for regenerating hair cells. Chicks were exposed in pairs to a 1500 Hz pure tone at 120 dBSPL for 18 h. The animals received repeated injections of 3H-thymidine over a survival period of 6, 15, or 24 h, 3 days or 30 days after the completion of noise exposure. One cochlea from each animal was processed for autoradiography and the other for scanning electron microscopy. Labeled, regenerated hair cells were present by 3 days after exposure and recovery from injury was nearly complete by 30 days. Examination of animals in short survival groups suggest that two precursor populations may exist. For inferior sensory epithelial damage, cuboidal or hyaline epithelial cells appear to serve as the precursor cell population for the regeneration of both hair cells and supporting cells. With isolated superior damage, however, supporting cells may be the precursor population.
利用氚化胸腺嘧啶核苷进行DNA标记，已记录了鸟类耳蜗受到声创伤后毛细胞的再生情况。本研究的目的是确定再生毛细胞的潜在前体细胞群。将雏鸡成对暴露于1500赫兹、120分贝声压级的纯音中18小时。在噪声暴露结束后的6小时、15小时、24小时、3天或30天的存活期内，给动物反复注射3H-胸腺嘧啶核苷。对每只动物的一个耳蜗进行放射自显影处理，另一个进行扫描电子显微镜检查。暴露后3天出现了标记的再生毛细胞，到30天时损伤恢复几乎完成。对短期存活组动物的检查表明可能存在两个前体细胞群。对于下感觉上皮损伤，立方形或透明上皮细胞似乎是毛细胞和支持细胞再生的前体细胞群。然而，对于孤立的上感觉上皮损伤，支持细胞可能是前体细胞群。
