Daubeuf François, Frossard Nelly
UMR 7200 CNRS-Université de Strasbourg, Laboratoire d'Innovation Thérapeutique, Faculté de Pharmacie, Illkirch, France.
Curr Protoc Mouse Biol. 2012 Jun 1;2(2):167-75. doi: 10.1002/9780470942390.mo110201.
Bronchoalveolar lavage (BAL) is a simple technique commonly used in humans to sample the contents of the epithelial lining fluid and determine the cellular and molecular composition of the pulmonary airways. In murine models, BAL makes it possible to sample immunological and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain reaction, and HPLC to assess such inflammatory components as cytokines, growth factors, analytes, and receptors expressed at the cell membrane. Performing BAL in a reproducible manner is a hallmark of airway research in the mouse. Several procedures may be implemented. This unit describes a basic, rapid, inexpensive, and highly reproducible procedure to collect BAL fluid and cells that can be counted efficiently and reproducibly. Curr. Protoc. Mouse Biol. 2:167-175 © 2012 by John Wiley & Sons, Inc.
支气管肺泡灌洗（BAL）是一种常用于人类的简单技术，用于采集上皮衬液的成分并确定肺气道的细胞和分子组成。在小鼠模型中，BAL能够对免疫和炎症细胞群体进行采样；对于研究气道疾病模型（如哮喘和慢性阻塞性肺疾病）中的细胞流入来说，它是必不可少的。细胞计数可以与酶联免疫吸附测定（ELISA）、免疫印迹、免疫组织化学、定量聚合酶链反应和高效液相色谱等方法相结合，以评估诸如细胞因子、生长因子、分析物和细胞膜上表达的受体等炎症成分。以可重复的方式进行BAL是小鼠气道研究的一个标志。可以实施几种操作程序。本单元描述了一种基本、快速、廉价且高度可重复的程序，用于收集能够高效且可重复计数的BAL液和细胞。《当代小鼠生物学实验指南》2：167 - 175 © 2012约翰威立父子出版公司
