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在体外试验中，二甲基亚砜会使顺铂对人髓性白血病细胞系的抗癌作用失活。

Dimethyl sulfoxide inactivates the anticancer effect of cisplatin against human myelogenous leukemia cell lines in in vitro assays.

作者信息

Raghavan Rahul, Cheriyamundath Sanith, Madassery Joseph

机构信息

Department of Biotechnology, University of Calicut, Malappuram, Kerala, India.

出版信息

Indian J Pharmacol. 2015 May-Jun;47(3):322-4. doi: 10.4103/0253-7613.157132.

DOI:10.4103/0253-7613.157132
PMID:26069372
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4450560/
Abstract

OBJECTIVES

To investigate the effect of DMSO on cisplatin induced cytotoxicity (invitro) against K562 (Human mylogenous leukemia) cell line and to study the cisplatin-DMSO adduct formation using UV-spectrophotometer.

MATERIALS AND METHODS

Effect of DMSO on the cytotoxicity of cisplatin was studied in K562 (Chronic mylogenous leukemia) cell line by MTT assay. Cisplatin-DMSO adduct formation was studied by continuously monitoring the increase in absorption peaks for 30 minutes using UV-spectrophotometer.

RESULTS

0.1-0.3% DMSO markedly reduced the cytotoxic activity of cisplatin in K562 cells. Cisplatin-DMSO adduct formation was detected using UV-spectrophotometer. Continuous increase in UV absorbance between 250nm-290nm was observed when cisplatin (0.5mg/ml) and DMSO (10%) were mixed.

CONCLUSION

Present study revealed that DMSO inactivates the cytotoxicity of cisplatin. Cisplatin-DMSO mixture showed increased absorbance at 250-290nm. Therefore, using DMSO in invitro assays might result in misinterpretation of actual efficacy of drugs.

摘要

目的

研究二甲基亚砜（DMSO）对顺铂在体外诱导的针对K562（人髓系白血病）细胞系的细胞毒性的影响，并使用紫外分光光度计研究顺铂 - DMSO加合物的形成。

材料与方法

通过MTT法研究DMSO对K562（慢性髓系白血病）细胞系中顺铂细胞毒性的影响。使用紫外分光光度计连续监测30分钟内吸收峰的增加，以研究顺铂 - DMSO加合物的形成。

结果

0.1 - 0.3%的DMSO显著降低了顺铂在K562细胞中的细胞毒性。使用紫外分光光度计检测到顺铂 - DMSO加合物的形成。当顺铂（0.5mg/ml）和DMSO（10%）混合时，观察到250nm - 290nm之间的紫外吸光度持续增加。

结论

本研究表明DMSO使顺铂的细胞毒性失活。顺铂 - DMSO混合物在250 - 290nm处显示吸光度增加。因此，在体外试验中使用DMSO可能会导致对药物实际疗效的错误解读。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d55/4450560/dcc1b473cdca/IJPharm-47-322-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d55/4450560/5751601b76c7/IJPharm-47-322-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d55/4450560/dcc1b473cdca/IJPharm-47-322-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d55/4450560/5751601b76c7/IJPharm-47-322-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d55/4450560/dcc1b473cdca/IJPharm-47-322-g002.jpg

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