Brotherton J
Department of Gynaecological Endocrinology, Sterility and Family Planning, Free University, Berlin, FRG.
Hum Reprod. 1989 Oct;4(7):837-42. doi: 10.1093/oxfordjournals.humrep.a136997.
Urinary human chorionic gonadotrophin (HCG) was estimated in 42 samples from patients in very early pregnancy or shortly after early spontaneous abortion, in which initial testing showed a positive urinary HCG in the Hybritech Tandem ICON II monoclonal 2 min test. There were no false positives with this test even when the blue colour was difficult to distinguish from the white background, i.e. considerably less than the calibration blue spot at 50 mIU/ml. The Serono polyclonal beta-HCG radioimmunoassay (RIA) detected urinary intact HCG (plus free beta-subunit) at concentrations as low as 10 mIU/ml IS 61/6 for bioassay (the old standard), i.e. approximately 22 mIU/ml 1st IRP 75/537 for immunoassay (the new standard). The LKB-Pharmacia DELFIA monoclonal fluoroimmunoassay (FIA) detected urinary intact HCG, without free beta-subunit, to a minimum concentration of 18 mIU/ml 75/537. The proportion of monoclonal/polyclonal was 2-155%. In normal amniotic fluid at 16-20 weeks of pregnancy, HCG concentrations were 3180 +/- 2270 (755-11,000) mIU/ml with the FIA.
对42份来自极早期妊娠患者或早期自然流产后不久患者的样本进行了尿人绒毛膜促性腺激素（HCG）检测，这些样本在Hybritech Tandem ICON II单克隆2分钟检测中初始检测显示尿HCG呈阳性。即使蓝色与白色背景难以区分，即远低于50 mIU/ml的校准蓝点，该检测也没有假阳性。赛诺诺公司的多克隆β-HCG放射免疫分析（RIA）检测尿中完整HCG（加上游离β亚基）的浓度低至10 mIU/ml（生物测定的IS 61/6，即旧标准），即免疫测定（新标准）中约为22 mIU/ml的第一国际参考品75/537。LKB-Pharmacia DELFIA单克隆荧光免疫分析（FIA）检测尿中完整HCG（无游离β亚基）的最低浓度为18 mIU/ml 75/537。单克隆/多克隆的比例为2 - 155%。在妊娠16 - 20周的正常羊水中，采用FIA检测时，HCG浓度为3180±2270（755 - 11,000）mIU/ml。
