Oumi Nao, Itamochi Hiroaki, Komatsu Hiroaki, Oishi Tetsuro, Shimada Muneaki, Sato Shinya, Chikumi Jun, Sato Seiya, Nonaka Michiko, Kudoh Akiko, Harada Tasuku
Department of Obstetrics and Gynecology, Tottori University School of Medicine, 36-1 Nishicho, Yonago, Tottori, 683-8504, Japan.
Hum Cell. 2016 Jan;29(1):46-51. doi: 10.1007/s13577-015-0120-8. Epub 2015 Jun 13.
A new cell line of human malignant peritoneal mesothelioma (MPM), TU-MM-1, was established and characterized. The cells showed polygonal morphology, grew in monolayers without contact inhibition and were arranged like a jigsaw puzzle. The chromosome numbers ranged from 41 to 44. A low rate of proliferation was observed and the doubling time was 67.9 h. Genomic DNA sequencing revealed that TU-MM-1 cells harbored missense mutations in APC, LATS2, BRCA1/2, and TP53, and mutation of a splice donor site in BAP1 and loss of CDKN2A gene. We observed the absence of BAP1 and p16(INK4a) proteins, underexpression of LATS2 protein, and overexpression of p53 protein in TU-MM-1 cells in western blot analysis. Heterotransplantation to nude mice produced tumors that had the characteristics of the original tumor. This cell line may be useful for studying biological properties and contribute to novel treatment strategies.
建立并鉴定了一种新的人恶性腹膜间皮瘤（MPM）细胞系TU-MM-1。这些细胞呈多边形形态，单层生长且无接触抑制，排列如拼图。染色体数目在41至44之间。观察到增殖率较低，倍增时间为67.9小时。基因组DNA测序显示，TU-MM-1细胞在APC、LATS2、BRCA1/2和TP53中存在错义突变，BAP1的剪接供体位点发生突变且CDKN2A基因缺失。在蛋白质印迹分析中，我们观察到TU-MM-1细胞中不存在BAP1和p16(INK4a)蛋白，LATS2蛋白表达不足，p53蛋白过表达。异种移植到裸鼠体内产生了具有原始肿瘤特征的肿瘤。该细胞系可能有助于研究生物学特性，并为新的治疗策略做出贡献。
