Singleton Timothy P, Yin Bin, Teferra Andinet, Mao Jenny Z
From the Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis;
Cyrus Tang Hematology Center, Jiangsu Institute of Hematology, the First Affiliated Hospital, Soochow University, Suzhou, China; and.
Am J Clin Pathol. 2015 Jul;144(1):137-44. doi: 10.1309/AJCPJ57YTEGLIUOI.
Clones of T-cell large granular lymphocytes (LGLTs) were detected by flow cytometry. Disease associations are described.
Flow cytometry on blood or marrow detected clonal LGLTs by analyzing variable regions of the T-cell receptor β chain.
LGLT clones were detected in 20% (54/264) of tested patients. The clone sizes were less than 2.0 × 10(9)/L in the blood in 73% and less than 10% of marrow space in 94%. Blood counts showed cytopenias. Clinical associations included B-cell clones, myeloid neoplasms, nonneoplastic disorders of blood or marrow, transplants, systemic immune disorders, carcinomas, or hypothyroidism. Twelve patients had LGLT leukemia. Most (76%) had small LGLT clones with limited impact on the clinical management.
Most of the LGLT clones detected by flow cytometry were small and did not change the clinical management. We propose the following terminology: T-cell clones of undetermined significance, LGLT leukemias, and T-cell immunoclones.
通过流式细胞术检测T细胞大颗粒淋巴细胞（LGLTs）克隆。描述疾病相关性。
对血液或骨髓进行流式细胞术，通过分析T细胞受体β链的可变区来检测克隆性LGLTs。
在20%（54/264）的受试患者中检测到LGLT克隆。血液中克隆大小在73%的患者中小于2.0×10⁹/L，在94%的患者中占骨髓空间不到10%。血细胞计数显示血细胞减少。临床相关性包括B细胞克隆、髓系肿瘤、血液或骨髓的非肿瘤性疾病、移植、全身性免疫疾病、癌症或甲状腺功能减退。12例患者患有LGLT白血病。大多数（76%）有小的LGLT克隆，对临床管理影响有限。
通过流式细胞术检测到的大多数LGLT克隆较小，并未改变临床管理。我们提出以下术语：意义未明的T细胞克隆、LGLT白血病和T细胞免疫克隆。
