Gao Li, Hu Yuejian, Wang Yuxia, Jiang Wenxin, He Zhiyan, Zhu Cailian, Ma Rui, Huang Zhengwei
Department of Endodontics, Ninth people's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, PR China.
Department of Endodontics, Ninth people's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, PR China.
Arch Oral Biol. 2015 Sep;60(9):1222-30. doi: 10.1016/j.archoralbio.2015.05.006. Epub 2015 May 23.
The aim of this article was to study the variation in oral microflora of the subgingival plaque during and after radiotherapy.
During and after radiotherapy, microbial samples were collected at seven time points (early stage, medium stage, and later stage of radiotherapy, and 1 month, 3 months, 6 months, and 1 year after radiotherapy) in three subjects for a total of 21 samples. Polymerase chain reaction (PCR) amplification was carried out on the 16S rDNA hypervariable V1-V3 region, and then the PCR products were determined by high-throughput pyrosequencing.
The rarefaction curve indicating the richness of the microflora demonstrated that the number of operational taxonomic units (OTUs) was in decline from the early stage of radiotherapy to the time point 1 month after radiotherapy and then trended upward. The Shannon diversity index declined during radiotherapy (ranging from 4.59 to 3.73), and generally rose after radiotherapy, with the lowest value of 3.5 (1 month after radiotherapy) and highest value of 4.75 (6 months after radiotherapy). A total of 120 genera were found; five genera (Actinomyces, Veillonella, Prevotella, Streptococcus, Campylobacter) were found in all subjects across all time points.
The richness and diversity of oral ecology decreased with increased radiation dose, and it was gradually restored with time.
本文旨在研究放疗期间及放疗后龈下菌斑口腔微生物群的变化。
在放疗期间及放疗后，对三名受试者在七个时间点（放疗早期、中期、后期以及放疗后1个月、3个月、6个月和1年）采集微生物样本，共21个样本。对16S rDNA高变V1 - V3区域进行聚合酶链反应（PCR）扩增，然后通过高通量焦磷酸测序对PCR产物进行测定。
表明微生物群丰富度的稀疏曲线显示，从放疗早期到放疗后1个月时，可操作分类单元（OTU）数量呈下降趋势，之后呈上升趋势。香农多样性指数在放疗期间下降（范围为4.59至3.73），放疗后总体上升，最低值为3.5（放疗后1个月），最高值为4.75（放疗后6个月）。共发现120个菌属；在所有受试者的所有时间点均发现五个菌属（放线菌属、韦荣球菌属、普雷沃菌属、链球菌属、弯曲杆菌属）。
口腔生态的丰富度和多样性随辐射剂量增加而降低，并随时间逐渐恢复。
