Chen K S, Quinnan G V
Division of Virology, Food and Drug Administration, Bethesda, Maryland 20892.
J Gen Virol. 1989 Dec;70 ( Pt 12):3291-6. doi: 10.1099/0022-1317-70-12-3291.
Parenteral immunization of BALB/c mice at 3 months of age with inactivated influenza virus vaccine elicited a haemagglutinin (HA)-specific serum IgG antibody response. The magnitude of this response declined with advancing age at the time of vaccination. By contrast, HA-specific IgA and IgG antibody levels observed in lung lavage fluids of mice immunized at 1 and 2 years of age were comparable to those of 5 month old mice when inactivated influenza virus vaccine was administered intragastrically. The secretory immune response was not fully developed in the first 3 weeks of life. However, the HA-specific IgA and IgG responses to oral vaccination in sera were reduced in 1 or 2 year old mice when compared to 5 month old mice. These data demonstrated the preservation of the virus-specific secretory IgA response in the pulmonary fluids of aged mice after oral vaccination with inactivated influenza virus vaccine. An age-dependent difference of systemic and mucosal immunity was evident in orally immunized mice.
3月龄的BALB/c小鼠经肠胃外途径用灭活流感病毒疫苗免疫后，引发了血凝素（HA）特异性血清IgG抗体反应。这种反应的强度在接种疫苗时会随着年龄的增长而下降。相比之下，当给1岁和2岁的小鼠经胃内途径接种灭活流感病毒疫苗时，在其肺灌洗液中观察到的HA特异性IgA和IgG抗体水平与5月龄小鼠相当。出生后的前3周，分泌性免疫反应尚未完全发育。然而，与5月龄小鼠相比，1岁或2岁小鼠血清中对口服疫苗的HA特异性IgA和IgG反应有所降低。这些数据表明，用灭活流感病毒疫苗口服免疫老年小鼠后，其肺液中病毒特异性分泌性IgA反应得以保留。在经口服免疫的小鼠中，全身免疫和黏膜免疫存在明显的年龄依赖性差异。
