Ohyama S, Yonemura Y, Miyazaki I
Department of Surgery 2nd, Kanazawa University School of Medicine, Japan.
Nihon Geka Gakkai Zasshi. 1989 Nov;90(11):1848-54.
Flow cytometric cell cycle analysis using a monoclonal antibody to Bromodeoxyuridine was performed on 117 gastric cancers. Dissociated cells were stained with indirect immunostaining for BrdU (FITC-BrdU) and propidium iodide. Bivariate BrdU/DNA distribution were obtained using EPICS-C flow cytometry. Tumor ploidy was classified as follows, D1: diploidy, D2: diploidy + aneuploidy, A1: single aneuploidy, A2: multiple aneuploidies. The ploidies of noncancerous gastric mucosa were all diploidy and that of S-phase fraction(SPF) were ranged from 0.0% to 1.2%. In 117 gastric cancers, aneuploidy was observed in 80 cases, D2: 38, A1: 15, A2: 27. SPF was higher in aneuploidy (14.5 +/- 5.1%) than diploidy (6.1 +/- 5.1%). Significant differences were observed between that of D1 (6.1 +/- 2.5) and D2 (13.6 +/- 6.2), A1 (12.5 +/- 4.5), A2 (16.0 +/- 3.2), and A1 and A2 (p less than 0.01). The patients with aneuploid tumors had poor prognosis than diploid tumors (p less than 0.05). In concerned with DNA ploidy pattern, the patients with A2 had most poor prognosis than the other (p less than 0.05). Furthermore, the patients with SPF over 10% had poor prognosis than that of SPF below 10%. These results indicated that DNA ploidy pattern and SPF may possibly be useful prognostic markers for gastric cancers.
使用抗溴脱氧尿苷单克隆抗体对117例胃癌进行流式细胞术细胞周期分析。解离后的细胞用间接免疫染色法进行BrdU（异硫氰酸荧光素标记的BrdU）和碘化丙啶染色。使用EPICS - C流式细胞仪获得双变量BrdU/DNA分布。肿瘤倍体分类如下，D1：二倍体，D2：二倍体+非整倍体，A1：单一非整倍体，A2：多个非整倍体。非癌性胃黏膜的倍体均为二倍体，S期分数（SPF）范围为0.0%至1.2%。在117例胃癌中，80例观察到非整倍体，D2：38例，A1：15例，A2：27例。非整倍体的SPF（14.5±5.1%）高于二倍体（6.1±5.1%）。D1（6.1±2.5）与D2（13.6±6.2）、A1（12.5±4.5）、A2（16.0±3.2）以及A1和A2之间观察到显著差异（p小于0.01）。非整倍体肿瘤患者的预后比二倍体肿瘤患者差（p小于0.05）。就DNA倍体模式而言，A2患者的预后比其他患者最差（p小于0.05）。此外，SPF超过10%的患者的预后比SPF低于10%的患者差。这些结果表明DNA倍体模式和SPF可能是胃癌有用的预后标志物。
