Sugai T, Uesugi N, Habano W, Nakamura S, Suto T, Fujimaki E, Itoh C
Division of Pathology, Central Clinical Laboratory, Iwate Medical University, Morioka, Japan.
Cytometry. 2000 Oct 15;42(5):270-6.
Although numerous studies of gastric cancers on DNA ploidy have been reported, differences in the degree of aneuploidy (DNA index, DI) during progression have not been identified. We attempted to chart the differences in DIs during progression to clarify the role of aneuploidy in gastric cancers. We classified the gastric cancers examined into intestinal (n = 88) and diffuse (n = 48) types, and then analyzed 136 gastric cancers (intramucosal cancer, 42; submucosal cancer, 39; advanced cancer, 55) by flow cytometry using multiple sampling. In addition, we examined the DNA ploidy pattern of mucosal and submucosal lesions using the same submucosal cancers to study the tumor progression in individual cancers. Intratumoral DNA differences in DNA ploidy were observed in both types of gastric cancers. In intestinal-type cancers, multiple subclones indicated by a different DI occurred during the early stage of gastric cancers, whereas in diffuse-type cancers, multiple subclones were found primarily in advanced cancers. Although the DI varied widely in early intestinal-type cancers between 1.0 and 2.0, in early diffuse-type cancers, the DI tended to be less than 1.2. However, in advanced stage gastric cancers, the DI distribution was similar for both histological types. In intestinal-type cancers, high DI (>1.3) aneuploidy was frequently found in mucosal lesions. In contrast, only low DI (<1.2) aneuploid clones were observed in mucosal lesions of diffuse-type cancers. The present results suggest that high DI aneuploid tumor clones in intramucosal cancers acquire invasive ability when they progress to submucosal cancers, whereas DNA aneuploidy itself plays an important role in submucosal invasion of diffuse-type cancers.
尽管已有众多关于胃癌DNA倍体的研究报道，但尚未明确其在进展过程中非整倍体程度（DNA指数，DI）的差异。我们试图描绘进展过程中DI的差异，以阐明非整倍体在胃癌中的作用。我们将所检查的胃癌分为肠型（n = 88）和弥漫型（n = 48），然后通过多次采样，运用流式细胞术分析了136例胃癌（黏膜内癌42例、黏膜下癌39例、进展期癌55例）。此外，我们利用相同的黏膜下癌来研究个体癌症的肿瘤进展，检测了黏膜和黏膜下病变的DNA倍体模式。在两种类型的胃癌中均观察到肿瘤内DNA倍体存在差异。在肠型癌中，胃癌早期阶段出现了由不同DI表示的多个亚克隆，而在弥漫型癌中，多个亚克隆主要在进展期癌中发现。尽管早期肠型癌的DI在1.0至2.0之间变化很大，但在早期弥漫型癌中，DI往往小于1.2。然而，在进展期胃癌中，两种组织学类型的DI分布相似。在肠型癌中，高DI（>1.3）非整倍体在黏膜病变中频繁出现。相比之下，在弥漫型癌的黏膜病变中仅观察到低DI（<1.2）非整倍体克隆。目前的结果表明，黏膜内癌中的高DI非整倍体肿瘤克隆在进展为黏膜下癌时获得侵袭能力，而DNA非整倍体本身在弥漫型癌的黏膜下浸润中起重要作用。
