Belkin V M, Tarasova E A, Dol'nikova A E
Mol Biol (Mosk). 1989 Sep-Oct;23(5):1364-72.
In our previous study the macromolecular complexes mostly consisting of fibronectin and procollagen were isolated from human fibroblast culture media using immobilized antibodies against fibronectin. At present an attempt was made to elucidate at what stage the formation of the fibronectin-collagen complex occurs--either in the course of incubation of the immobilized antibodies with labelled proteins secreted by fibroblasts, or in the extracellular space while labelling fibroblasts, or intracellularly. The results obtained show that the fibronectin-collagen complex: 1) pre-exists even before incubation with the immobilized antibodies and 2) it is of intracellular origin. Thus, the considerable amount of the fibroblast-secreted fibronectin (no less than 20%) is released from the cell not in the free form but in the complex with procollagen. It was suggested that the fibronectin-collagen complex presents a stage in the formation of the insoluble extracellular matrix.
在我们之前的研究中，主要由纤连蛋白和前胶原组成的大分子复合物是使用针对纤连蛋白的固定化抗体从人成纤维细胞培养基中分离出来的。目前，我们试图阐明纤连蛋白 - 胶原蛋白复合物的形成发生在哪个阶段——是在固定化抗体与成纤维细胞分泌的标记蛋白孵育过程中，还是在标记成纤维细胞时的细胞外空间中，抑或是在细胞内。获得的结果表明，纤连蛋白 - 胶原蛋白复合物：1）甚至在与固定化抗体孵育之前就已预先存在，2）其起源于细胞内。因此，相当数量的成纤维细胞分泌的纤连蛋白（不少于20%）不是以游离形式从细胞中释放出来，而是与前胶原形成复合物释放出来。有人提出，纤连蛋白 - 胶原蛋白复合物是不溶性细胞外基质形成过程中的一个阶段。
