Belkin V M, Dol'nikova A E, Volodarskaia S M, Mazurov V I
Vopr Med Khim. 1988 Jul-Aug;34(4):101-4.
Interaction of labelled proteins from human fibroblast culture and the antibodies to fibronectin, which were used as soluble and Sepharose-immobilized preparations, was studied. Electrophoretic mobilities of the proteins bound to antibodies were compared. Soluble antibodies bound only fibronectin, while immobilized antibodies bound, except for fibronectin, other low molecular proteins. After treatment of the cultural proteins with bacterial collagenase two main components, besides fibronectin, were eliminated, thus suggesting their collagen-like nature. Two dimers of fibronectin per one trimer of procollagen were present in the material bound to immobilized antibodies to fibronectin. Control studies demonstrated that the data obtained were not an artefact occurring due to sorption of proteins on Sepharose or to unspecific actions of antibodies.
研究了来自人成纤维细胞培养物的标记蛋白与用作可溶性和琼脂糖固定化制剂的纤连蛋白抗体之间的相互作用。比较了与抗体结合的蛋白的电泳迁移率。可溶性抗体仅结合纤连蛋白，而固定化抗体除了结合纤连蛋白外，还结合其他低分子蛋白。用细菌胶原酶处理培养蛋白后，除纤连蛋白外的两个主要成分被去除，这表明它们具有胶原样性质。在与纤连蛋白固定化抗体结合的物质中，每一个原胶原三聚体存在两个纤连蛋白二聚体。对照研究表明，所获得的数据不是由于蛋白吸附在琼脂糖上或抗体的非特异性作用而产生的假象。
