Tang Yu, Hu Chao, Liao Qiong, Liu Wen-long, Yang Yan-tao, He Hong, He Fu-yuan
Zhongguo Zhong Yao Za Zhi. 2015 Jan;40(2):240-4.
The solubility parameter determination of astrageloside from Buyang Huanwu decoction with inverse gas chromatography (IGC) method evaluation was investigated in this paper. Di-n-octyl phthalate Kwai alternative sample was used to carry out methodological study. The accuracy of the measured correlation coefficient was 0.992 1. Experimental precision measured by IGC experiments showed that the results were accurate and reliable. The sample was uniformly coated on the surface of an inert carrier and N2 gas was carrier gas, a variety of polar solvents such as isopropanol, toluene, acetone, chloroform, cyclohexane as probes. TCD detector temperature was 150 degrees C, gas room temperature was 120 degrees C. Similar headspace method was used whichever over 1 μL gas into the GC measurement, Retention time t(R), t(0) and all the parameters of air and probes molecules within the column were tested. Astragaloside solubility parameter was (21.02 ± 2.4) [J x cm(-3)] ½, literature value was 19.24 [J x cm(-3)] ½, and relevant coefficient was 0.984 5. IGC method is effective and accurate to measure ingredients solubility parameter.
本文研究了采用反相气相色谱（IGC）法评估补阳还五汤中黄芪甲苷的溶解度参数。以邻苯二甲酸二正辛酯替代样品进行方法学研究。测得的相关系数准确性为0.992 1。通过IGC实验测得的实验精密度表明结果准确可靠。将样品均匀涂覆在惰性载体表面，以N₂气为载气，以异丙醇、甲苯、丙酮、氯仿、环己烷等多种极性溶剂作为探针。热导检测器温度为150℃，气室温度为120℃。采用类似顶空进样法进样超过1μL气体进行气相色谱测定，测试保留时间t(R)、t(0)以及柱内空气和探针分子的所有参数。黄芪甲苷的溶解度参数为(21.02±2.4)[J·cm⁻³]½，文献值为19.24[J·cm⁻³]½，相关系数为0.984 5。IGC法用于测定成分溶解度参数有效且准确。
