Cho Joon Hyeong, Kwon Jung Eun, Cho Youngmi, Kim Inhye, Kang Se Chan
Department of Biological and Environmental Science, Dongguk University, Goyang 410-820, Korea.
Department of Biological Science, Gachon University, Seongnam 461-701, Korea.
Nutrients. 2015 Jun 15;7(6):4862-74. doi: 10.3390/nu7064862.
Angelica gigas (AG) is effective against various medical conditions such as bacterial infection, inflammation, and cancer. It contains a number of coumarin compounds and the group of interest is the pyranocoumarin, which comprises decursin and decursinol angelate. This group has an effect on controlling inflammation, which is caused by excessive nitric oxide (NO) production. Heme oxygenases (HOs), particularly HO-1, play a role in regulating the production of NO. Thus, this study aimed to investigate the anti-inflammatory effects of AG by measuring HO-1 expression. Treatments with CH2Cl2 layer and Angelica gigas extract (AGE) showed the highest NO inhibition effects. Decursin, decursinol angelate, and nodakenin were isolated from the CH2Cl2 layer of AGE. Decursin also demonstrated the highest anti-oxidative effect among the coumarins. Although decursin had the best NO inhibition and anti-oxidative effects, the effects of AGE treatment far surpassed that of decursin. This is owing to the combination effect of the coumarins present within AGE, which is a solvent extract of AG. The expression of HO-1 is an effective indicator of the anti-inflammatory effects of AG. Based on the results of the coumarin compounds, HO-1 expression was found to be dose dependent and specific to decursin.
东当归（AG）对多种病症有效，如细菌感染、炎症和癌症。它含有多种香豆素化合物，其中感兴趣的一类是吡喃香豆素，包括蛇床子素和当归醇蛇床子酸酯。该类化合物对控制由过量一氧化氮（NO）产生引起的炎症有作用。血红素加氧酶（HOs），特别是HO-1，在调节NO的产生中起作用。因此，本研究旨在通过测量HO-1表达来研究AG的抗炎作用。用二氯甲烷层和东当归提取物（AGE）处理显示出最高的NO抑制作用。从AGE的二氯甲烷层中分离出蛇床子素、当归醇蛇床子酸酯和紫花前胡苷。在香豆素中，蛇床子素也表现出最高的抗氧化作用。尽管蛇床子素具有最佳的NO抑制和抗氧化作用，但AGE处理的效果远远超过蛇床子素。这是由于AGE中存在的香豆素的联合作用，AGE是AG的溶剂提取物。HO-1的表达是AG抗炎作用的有效指标。基于香豆素化合物的结果，发现HO-1表达呈剂量依赖性且对蛇床子素具有特异性。
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