Kim Mi Hye, Lee Haesu, Yang Woong Mo
Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University Seoul 02447 Republic of Korea
RSC Adv. 2018 Apr 9;8(24):13089-13093. doi: 10.1039/c7ra12531c.
(AG) has been used for periodontal diseases in traditional Korean medicine. However, the effects of AG on periodontitis have not been clarified yet. In this study, we investigated the ameliorative effects of AG against ligature-induced periodontitis. Sprague-Dawley rats were divided into four groups; non-ligatured (normal), ligatured and treated with vehicle (ligatured), ligatured and treated with 1 mg mL AG (AG1), and ligatured and treated with 100 mg mL AG (AG100). 70% ethanol extracts of AG were topically applied onto both sides of the first molar daily for 14 days. In addition, human dermal fibroblast cells were treated with 1, 10 and 100 μg mL AG to characterize the expression of matrix metallopeptidase 9 (MMP-9). Topical AG treatment reduced alveolar bone resorption, as assessed by methylene blue staining. The structures of soft gingival tissues (periodontal pocket) were recovered in the AG-treated groups. The expression of MMP-9 was decreased, and that of type 1 collagen was significantly increased in AG-treated gingival tissues. In addition, AG treatment inhibited the activity of MMP-9 in LPS-treated human dermal fibroblast cells. This study reveals that topical AG treatment has the potential to ameliorate the destruction of gingival tissues by inhibiting MMP-9 activity. AG may be a candidate for the treatment of periodontitis.
在传统韩医学中，（AG）已被用于治疗牙周疾病。然而，AG对牙周炎的影响尚未明确。在本研究中，我们调查了AG对结扎诱导的牙周炎的改善作用。将Sprague-Dawley大鼠分为四组：未结扎（正常）组、结扎并用赋形剂处理（结扎）组、结扎并用1mg/mL AG处理（AG1）组和结扎并用100mg/mL AG处理（AG100）组。每天将AG的70%乙醇提取物局部应用于第一磨牙两侧，持续14天。此外，用人皮肤成纤维细胞分别用1、10和100μg/mL AG处理，以表征基质金属蛋白酶9（MMP-9）的表达。通过亚甲蓝染色评估，局部应用AG治疗可减少牙槽骨吸收。在AG治疗组中，牙龈软组织（牙周袋）的结构得以恢复。在AG处理的牙龈组织中，MMP-9的表达降低，而I型胶原蛋白的表达显著增加。此外，AG处理抑制了脂多糖处理的人皮肤成纤维细胞中MMP-9的活性。本研究表明，局部应用AG治疗有可能通过抑制MMP-9活性来改善牙龈组织的破坏。AG可能是治疗牙周炎的候选药物。
