Saneyoshi M, Hasobe M, Yamamoto K
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Nucleic Acids Symp Ser. 1989(21):49-50.
A series of 9-beta-D-ribofuranosyl-6-alkylthiopurines (6-alkyl TI) were found to inhibit in vitro replication of infectious hematopoietic necrosis virus (IHNV), human influenza virus (IFV) and respiratory syncytial virus (RSV) with IC50 values of about 0.06 microgram/ml, 0.7-1.5 micrograms/ml and 1-3 micrograms/ml, respectively. Viral RNA synthesis in infected cells in the presence of actinomycin D was inhibited by treatment with the compounds dose-dependently. It was also found that the decrease of rNTP pool size in infected cells was remarkably dose-dependent. From these findings, the mode of antiviral action of these compounds may be explained by rNTP imbalance in the treated group.
一系列9-β-D-呋喃核糖基-6-烷基硫代嘌呤（6-烷基TI）被发现可抑制传染性造血坏死病毒（IHNV）、人流感病毒（IFV）和呼吸道合胞病毒（RSV）的体外复制，其IC50值分别约为0.06微克/毫升、0.7 - 1.5微克/毫升和1 - 3微克/毫升。在用放线菌素D存在的情况下，用这些化合物处理可剂量依赖性地抑制感染细胞中的病毒RNA合成。还发现感染细胞中rNTP库大小的减少明显呈剂量依赖性。从这些发现来看，这些化合物的抗病毒作用模式可能是由处理组中的rNTP失衡来解释的。
