†School of Chemistry, the Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv 69978, Israel.
‡The Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv 69978, Israel.
Nano Lett. 2015 Jul 8;15(7):4758-68. doi: 10.1021/acs.nanolett.5b01578. Epub 2015 Jun 30.
Significant research efforts have been dedicated to the integration of biological species with electronic elements to yield smart bioelectronic devices. The integration of DNA, proteins, and whole living cells and tissues with electronic devices has been developed into numerous intriguing applications. In particular, the quantitative detection of biological species and monitoring of biological processes are both critical to numerous areas of medical and life sciences. Nevertheless, most current approaches merely focus on the "monitoring" of chemical processes taking place on the sensing surfaces, and little efforts have been invested in the conception of sensitive devices that can simultaneously "control" and "monitor" chemical and biological reactions by the application of on-surface reversible stimuli. Here, we demonstrate the light-controlled fine modulation of surface pH by the use of photoactive molecularly modified nanomaterials. Through the use of nanowire-based FET devices, we showed the capability of modulating the on-surface pH, by intensity-controlled light stimulus. This allowed us simultaneously and locally to control and monitor pH-sensitive biological reactions on the nanodevices surfaces, such as the local activation and inhibition of proteolytic enzymatic processes, as well as dissociation of antigen-antibody binding interactions. The demonstrated capability of locally modulating the on-surface effective pH, by a light stimuli, may be further applied in the local control of on-surface DNA hybridization/dehybridization processes, activation or inhibition of living cells processes, local switching of cellular function, local photoactivation of neuronal networks with single cell resolution and so forth.
大量的研究致力于将生物物种与电子元件集成，以产生智能生物电子设备。将 DNA、蛋白质和整个活细胞和组织与电子设备集成已经开发出了许多有趣的应用。特别是，对生物物种的定量检测和对生物过程的监测对医学和生命科学的许多领域都至关重要。然而，目前大多数方法仅仅关注于在传感表面上发生的化学过程的“监测”，而很少有精力投入到可以通过表面可逆刺激的应用同时“控制”和“监测”化学和生物反应的敏感设备的构思中。在这里，我们展示了通过光活性分子修饰的纳米材料来精细调控表面 pH 值。通过使用基于纳米线的 FET 器件，我们展示了通过强度控制的光刺激来调制表面 pH 值的能力。这使我们能够同时和局部地控制和监测纳米器件表面上的 pH 敏感生物反应，例如局部激活和抑制蛋白水解酶过程，以及抗原-抗体结合相互作用的解离。通过光刺激局部调制表面有效 pH 值的能力可以进一步应用于表面 DNA 杂交/解杂交过程的局部控制、活细胞过程的激活或抑制、细胞功能的局部切换、单个细胞分辨率的神经元网络的局部光激活等。
