古柯叶中的成分I：秘鲁三个地区可卡因的气相色谱分析

Constituents in Erythroxylum coca I: gas chromatographic analysis of cocaine from three locations in Peru.

作者信息

Turner C E, Ma C Y, Elsohly M A

出版信息

Bull Narc. 1979 Jan-Mar;31(1):71-6.

Abstract

A method for the determination of cocaine content in coca leaves (Erythroxylum coca) has been developed. The procedure involves refluxing the powdered leaves in 95% ethanol for 15 minutes, followed by acid-base partitioning with chloroform and a GLC assay. The recovery of cocaine was quantitative. This procedure was applied to determine cocaine content in three samples of Erythroxylum coca Lam. collected from different geographic locations in Peru. Using androst-4-ene-3,17-dione as the internal standard the calibration curve was linear over a factor of 0.5 to 10 fold cocaine concentration relative to internal standard. The slope (b) was 0.733, the coefficient of determination (r2) was 1.00 and the average precision was 3.9%.

摘要

已开发出一种测定古柯叶（Erythroxylum coca）中可卡因含量的方法。该程序包括将粉末状叶片在95%乙醇中回流15分钟，然后用氯仿进行酸碱分配并进行气相色谱分析。可卡因的回收率是定量的。该程序用于测定从秘鲁不同地理位置采集的三个古柯叶（Erythroxylum coca Lam.）样品中的可卡因含量。以雄甾-4-烯-3,17-二酮为内标，相对于内标，校准曲线在可卡因浓度为0.5至10倍的范围内呈线性。斜率（b）为0.733，决定系数（r2）为1.00，平均精密度为3.9%。