McCampbell Kristen K, Springer Kristin N, Wingert Rebecca A
Department of Biological Sciences and Center for Zebrafish Research, University of Notre Dame, Notre Dame, IN 46556, USA.
Stem Cells Int. 2015;2015:547636. doi: 10.1155/2015/547636. Epub 2015 May 18.
The zebrafish is a useful animal model to study the signaling pathways that orchestrate kidney regeneration, as its renal nephrons are simple, yet they maintain the biological complexity inherent to that of higher vertebrate organisms including mammals. Recent studies have suggested that administration of the aminoglycoside antibiotic gentamicin in zebrafish mimics human acute kidney injury (AKI) through the induction of nephron damage, but the timing and details of critical phenotypic events associated with the regeneration process, particularly in existing nephrons, have not been characterized. Here, we mapped the temporal progression of cellular and molecular changes that occur during renal epithelial regeneration of the proximal tubule in the adult zebrafish using a platform of histological and expression analysis techniques. This work establishes the timing of renal cell death after gentamicin injury, identifies proliferative compartments within the kidney, and documents gene expression changes associated with the regenerative response of proliferating cells. These data provide an important descriptive atlas that documents the series of events that ensue after damage in the zebrafish kidney, thus availing a valuable resource for the scientific community that can facilitate the implementation of zebrafish research to delineate the mechanisms that control renal regeneration.
斑马鱼是一种有用的动物模型，可用于研究协调肾脏再生的信号通路。因为其肾单位简单，但仍保留了包括哺乳动物在内的高等脊椎动物所固有的生物学复杂性。最近的研究表明，在斑马鱼中施用氨基糖苷类抗生素庆大霉素可通过诱导肾单位损伤来模拟人类急性肾损伤（AKI），但与再生过程相关的关键表型事件的时间和细节，特别是在现有肾单位中的情况，尚未得到描述。在这里，我们使用组织学和表达分析技术平台，绘制了成年斑马鱼近端小管肾上皮再生过程中发生的细胞和分子变化的时间进程。这项工作确定了庆大霉素损伤后肾细胞死亡的时间，识别了肾脏内的增殖区室，并记录了与增殖细胞再生反应相关的基因表达变化。这些数据提供了一个重要的描述图谱，记录了斑马鱼肾脏损伤后发生的一系列事件，从而为科学界提供了一个宝贵的资源，有助于开展斑马鱼研究以阐明控制肾脏再生的机制。
