基于质谱的蛋白质组学研究使高密度脂蛋白成为动脉粥样硬化性血管疾病的生物标志物。
Mass Spectrometry-Based Proteomic Study Makes High-Density Lipoprotein a Biomarker for Atherosclerotic Vascular Disease.
作者信息
Chang Chiz-Tzung, Yang Chao-Yuh, Tsai Fuu-Jen, Lin Shih-Yi, Chen Chao-Jung
机构信息
College of Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan ; Division of Nephrology, China Medical University Hospital, 2 Yu-Der Road, Taichung 40447, Taiwan ; L5 Research Center, China Medical University Hospital, Taichung, Taiwan.
L5 Research Center, China Medical University Hospital, Taichung, Taiwan ; Section of Cardiovascular Research, Department of Medicine, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
出版信息
Biomed Res Int. 2015;2015:164846. doi: 10.1155/2015/164846. Epub 2015 May 18.
High-density lipoprotein (HDL) is a lipid and protein complex that consists of apolipoproteins and lower level HDL-associated enzymes. HDL dysfunction is a factor in atherosclerosis and decreases patient survival. Mass spectrometry- (MS-) based proteomics provides a high throughput approach for analyzing the composition and modifications of complex HDL proteins in diseases. HDL can be separated according to size, surface charge, electronegativity, or apoprotein composition. MS-based proteomics on subfractionated HDL then allows investigation of lipoprotein roles in diseases. Herein, we review recent developments in MS-based quantitative proteomic techniques, HDL proteomics and lipoprotein modifications in diseases, and HDL subfractionation studies. We also discuss future directions and perspectives in MS-based proteomics on HDL.
高密度脂蛋白（HDL）是一种脂质与蛋白质的复合物，由载脂蛋白和较低水平的HDL相关酶组成。HDL功能障碍是动脉粥样硬化的一个因素，并会降低患者生存率。基于质谱（MS）的蛋白质组学为分析疾病中复杂HDL蛋白质的组成和修饰提供了一种高通量方法。HDL可根据大小、表面电荷、电负性或载脂蛋白组成进行分离。然后，对亚组分HDL进行基于MS的蛋白质组学研究，有助于探究脂蛋白在疾病中的作用。在此，我们综述了基于MS的定量蛋白质组学技术、HDL蛋白质组学以及疾病中脂蛋白修饰和HDL亚组分研究的最新进展。我们还讨论了基于MS的HDL蛋白质组学的未来方向和前景。
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