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急性冠状动脉综合征重塑高密度脂蛋白亚组分的蛋白质成分及功能。

Acute coronary syndrome remodels the protein cargo and functions of high-density lipoprotein subfractions.

作者信息

Tan Ying, Liu Ting Rong, Hu Shui Wang, Tian Di, Li Chen, Zhong Jian Kai, Sun Hai Ge, Luo Tian Tian, Lai Wen Yan, Guo Zhi-Gang

机构信息

Division of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, P.R. China.

Laboratory of Pathophysiology, Southern Medical University, Guangzhou, Guangdong, P.R. China.

出版信息

PLoS One. 2014 Apr 15;9(4):e94264. doi: 10.1371/journal.pone.0094264. eCollection 2014.

DOI:10.1371/journal.pone.0094264
PMID:24736723
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3988065/
Abstract

OBJECTIVES

This study examined alterations in the functions and proteome of high-density lipoprotein (HDL) subfractions (HDL2 and HDL3) isolated from patients with acute coronary syndrome (ACS) compared with control subjects.

METHODS

We measured HDL subfraction cholesterol efflux capacity, inflammatory index (HII), paraoxonase-1 (PON1) activity, and lipid hydroperoxide (LOOH) levels in both male age-matched controls and the ACS group (n = 40/group). Additionally, proteomic analysis was used to monitor changes in the HDL subfraction proteome between controls and ACS subjects.

RESULTS

Both HDL2 and HDL3 from ACS patients had greater HII and LOOH levels compared with controls (P<0.001); PON1 activity and cholesterol efflux capacity in both HDL2 and HDL3 from the ACS group were significantly less than those of controls (P<0.001). Using proteomic analysis, we demonstrated that, compared with the control group, nine proteins were selectively enriched in HDL3 from subjects with ACS, and ras-related protein Rab-7b was decreased in HDL3. Additionally, in the ACS subjects, 12 proteins were decreased in HDL2 and 4 proteins were increased in HDL2.

CONCLUSIONS

Functional HDL subfractions shifted to dysfunctional HDL subfractions during ACS, and the functional impairment was linked to remodeled protein cargo in HDL subfractions from ACS patients.

摘要

目的

本研究检测了与对照受试者相比，从急性冠状动脉综合征（ACS）患者中分离出的高密度脂蛋白（HDL）亚组分（HDL2和HDL3）的功能和蛋白质组变化。

方法

我们测量了年龄匹配的男性对照组和ACS组（每组n = 40）的HDL亚组分胆固醇流出能力、炎症指数（HII）、对氧磷酶-1（PON1）活性和脂质过氧化氢（LOOH）水平。此外，蛋白质组分析用于监测对照组和ACS受试者之间HDL亚组分蛋白质组的变化。

结果

与对照组相比，ACS患者的HDL2和HDL3均具有更高的HII和LOOH水平（P<0.001）；ACS组HDL2和HDL3中的PON1活性和胆固醇流出能力均显著低于对照组（P<0.001）。通过蛋白质组分析，我们证明，与对照组相比，ACS受试者的HDL3中有9种蛋白质选择性富集，而HDL3中的ras相关蛋白Rab-7b减少。此外，在ACS受试者中，HDL2中有12种蛋白质减少，HDL2中有4种蛋白质增加。

结论

在ACS期间，功能性HDL亚组分转变为功能失调的HDL亚组分，并且功能损害与ACS患者HDL亚组分中重塑的蛋白质载量有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c11/3988065/225b2ae29d29/pone.0094264.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c11/3988065/bf1cc17806ed/pone.0094264.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c11/3988065/2cfd84edde1b/pone.0094264.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c11/3988065/bb1104f99485/pone.0094264.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c11/3988065/7535bb9d4d52/pone.0094264.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c11/3988065/225b2ae29d29/pone.0094264.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c11/3988065/bf1cc17806ed/pone.0094264.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c11/3988065/2cfd84edde1b/pone.0094264.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c11/3988065/bb1104f99485/pone.0094264.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c11/3988065/7535bb9d4d52/pone.0094264.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6c11/3988065/225b2ae29d29/pone.0094264.g005.jpg

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