Borgie Mireille, Dagher Zeina, Ledoux Frédéric, Verdin Anthony, Cazier Fabrice, Martin Perrine, Hachimi Adam, Shirali Pirouz, Greige-Gerges Hélène, Courcot Dominique
Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), EA 4492, Maison de la Recherche en Environnement Industriel, Université du Littoral Côte d'Opale, 145 Avenue Maurice Schumann, 59140 Dunkerque, France; Bioactive Molecules Research Group, Doctoral School of Sciences and Technologies, Lebanese University, Beirut, Lebanon.
Bioactive Molecules Research Group, Doctoral School of Sciences and Technologies, Lebanese University, Beirut, Lebanon; Department of Biology, Faculty of Sciences-2, Lebanese University, Beirut, Lebanon.
Environ Pollut. 2015 Oct;205:250-60. doi: 10.1016/j.envpol.2015.05.027. Epub 2015 Jun 18.
During the last few years, the induction of toxicological mechanisms by atmospheric ultrafine particles (UFP) has become one of the most studied topics in toxicology and a subject of huge debates. Fine particles (FP) and UFP collected at urban and rural sites in Lebanon were studied for their chemical composition and toxicological effects. UFP were found more enriched in trace elements, secondary inorganic ions, total carbon and organic compounds than FP. For toxicological analysis, BEAS-2B cells were exposed for 24, 48 and 72 h to increasing concentrations of FP, water-UFP suspension (UFPw) and UFP organic extract (UFPorg). Our findings showed that UFP caused earlier alterations of mitochondrial metabolism and membrane integrity from the lowest concentrations. Moreover, a significant induction of CYP1A1, CYP1B1 and AhRR genes expression was showed after cells exposure to UFPorg and to a lesser extent to UFPw and FP samples.
在过去几年中，大气超细颗粒物（UFP）诱导毒理学机制已成为毒理学领域研究最多的课题之一，也是激烈争论的主题。对在黎巴嫩城乡地区采集的细颗粒物（FP）和超细颗粒物进行了化学成分和毒理学效应研究。发现超细颗粒物比细颗粒物在微量元素、二次无机离子、总碳和有机化合物方面含量更高。为进行毒理学分析，将BEAS-2B细胞暴露于浓度不断增加的细颗粒物、水-超细颗粒物悬浮液（UFPw）和超细颗粒物有机提取物（UFPorg）中24、48和72小时。我们的研究结果表明，超细颗粒物从最低浓度就会导致线粒体代谢和膜完整性的早期改变。此外，细胞暴露于超细颗粒物有机提取物后，CYP1A1、CYP1B1和AhRR基因表达显著上调，暴露于水-超细颗粒物悬浮液和细颗粒物样品后上调程度较小。
