Kulikowska-Karpińska Elżbieta, Czerw Katarzyna
Wiad Lek. 2015;68(1):32-8.
Cigarette smoking is one of the most common habits of the modern world. The World Health Organization (WHO) showed that about 30% of the world's population over the age of 15 smoked cigarettes. That gives the number of 1.1 billion smokers. It is known that the habit of smoking is a serious risk to human health and life. Cigarette smoke induces the formation of reactive oxygen species (ROS). ROS can cause direct damage of DNA chain and leads to the mutagenic and carcinogenic effect.
To evaluate the concentration of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the urine of smokers. Based on the obtained results an attempt to determine whether the concentration of 8-OH-dG in the urine can be an indicator of genotoxicity of tobacco smoke.
The study was conducted in 83 healthy volunteers, 63 smokers and 20 non-smokers (control group). The age of patients in both groups were in the range 20-60 years. Average age of women and men in the control group and the test group was 34 years. Duration of smoking was 1-30 years, the number of cigarettes smoked from 1 to over 20 per day. In the urine the concentration of 8-OH-dG was determined using Bioxytech® 8-OH-dG-EIA™Kit (manufactured by OXIS International, Inc., Portland, USA) and the concentration of creatinine using in vitro diagnostic kit BIOLABO. The concentration of 8-OHdG in urine was expressed in ng/g creatinine.
More than 3-fold increase in the concentration of 8-OHdG in the urine of smokers was found. The increase in the concentration of 8-OHdG in the urine depended on age, the amount of smoked cigarettes per 24 hours, duration of smoking and cigarette brands.
The increase in the concentration of 8-OHdG in the urine of smokers, both women and men indicates DNA damage and the genotoxicity action of cigarette smoke. According to our previously conducted studies and examinations of other authors, it can be suggested that the concentration of 8-OHdG in urine may be considered as the sensitive and specific marker of oxidative DNA damage.
吸烟是现代社会最常见的习惯之一。世界卫生组织（WHO）表明，全球15岁以上人口中约30%吸烟，吸烟者数量达11亿。众所周知，吸烟习惯对人类健康和生命构成严重风险。香烟烟雾会诱导活性氧（ROS）的形成。ROS可直接损伤DNA链，导致诱变和致癌作用。
评估吸烟者尿液中8-羟基-2'-脱氧鸟苷（8-OHdG）的浓度。基于所得结果，尝试确定尿液中8-OH-dG的浓度是否可作为烟草烟雾遗传毒性的指标。
研究对象为83名健康志愿者，其中63名吸烟者和20名非吸烟者（对照组）。两组患者年龄均在20至60岁之间。对照组和试验组中女性和男性的平均年龄均为34岁。吸烟时长为1至30年，每日吸烟量从1支至20支以上不等。使用Bioxytech® 8-OH-dG-EIA™试剂盒（由美国波特兰的OXIS International, Inc.生产）测定尿液中8-OH-dG的浓度，使用体外诊断试剂盒BIOLABO测定肌酐浓度。尿液中8-OHdG的浓度以ng/g肌酐表示。
吸烟者尿液中8-OHdG的浓度增加了3倍多。尿液中8-OHdG浓度的增加取决于年龄、24小时吸烟量、吸烟时长和香烟品牌。
吸烟者尿液中8-OHdG浓度的增加，无论男女，均表明DNA损伤以及香烟烟雾的遗传毒性作用。根据我们之前进行的研究以及其他作者的研究，可以认为尿液中8-OHdG的浓度可被视为氧化DNA损伤的敏感且特异的标志物。
