Shen Kang-Ning, Chang Chih-Wei, Chen Ching-Hung, Hsiao Chung-Der
a Center of Excellence for the Oceans, National Taiwan Ocean University , Keelung, Taiwan , ROC .
b National Museum of Marine Biology and Aquarium , Pingtung, Taiwan , ROC .
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2807-8. doi: 10.3109/19401736.2015.1053077. Epub 2015 Jun 22.
Multicolor angelfish (Centropyge multicolor) and Yellowhead angelfish (C. joculator) are in the "multicolor species complex". In this study, we used the next-generation sequencing method to deduce two complete mitogenomes of C. multicolor and C. joculator for the first time. The assembled mitogenome, consisting of 16,791 bp and 16,778 bp, showing 99% identity to each other. Both mitogenome follow the typical vertebrate arrangement, including 13 protein coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes, and a non-coding control region of D-loop. D-loop contains 909 bp in C. multicolor and 896 bp in C. joculator and locates between tRNA-Pro and tRNA-Phe. The overall GC content is 46.9% for C. multicolor and 50.7% for C. joculator. Complete mitogenomes of C. multicolor and C. joculator provide essential and important DNA molecular data for phylogenetic and evolutionary analysis for "multicolor species complex".
五彩神仙鱼（Centropyge multicolor）和黄头神仙鱼（C. joculator）属于“五彩物种复合体”。在本研究中，我们首次使用下一代测序方法推导得出五彩神仙鱼和黄头神仙鱼的两个完整线粒体基因组。组装后的线粒体基因组分别由16,791 bp和16,778 bp组成，彼此间具有99%的同一性。两个线粒体基因组均遵循典型的脊椎动物排列方式，包括13个蛋白质编码基因、22个转运RNA、2个核糖体RNA基因以及一个D环非编码控制区。五彩神仙鱼的D环包含909 bp，黄头神仙鱼的D环包含896 bp，位于tRNA-Pro和tRNA-Phe之间。五彩神仙鱼的总体GC含量为46.9%，黄头神仙鱼的总体GC含量为50.7%。五彩神仙鱼和黄头神仙鱼的完整线粒体基因组为“五彩物种复合体”的系统发育和进化分析提供了重要的DNA分子数据。
