Iamnikova S S, Kovtun T O, Dmitriev G A, Shemiakin I G, Semenova N P, L'vov D K, Chambers T, Vebster R
Vopr Virusol. 1989 Sep-Oct;34(5):568-72.
The antigenic structure of influenza H13 viruses isolated from wild birds in the USSR in 1976-1985 was studied. Antiserum against the reference A/gull/Maryland/704/77 (H13N6) strain was used to demonstrate the antigenic variations among the viruses. The homology of nucleotide sequences in the region 99-215 for the two A/H13N6 strains, A/gull/Maryland/704/77 and A/great black-headed gull/Astrakhan/227/84, were 75% and 86%, respectively. The 9-base segment deletion in A/grey black-headed gull/Astrakhan/277/84 was observed. Comparison of the predicted amino acid sequences of the strains' hemagglutinin in the appropriate region (amino acids 2-40) revealed 5 replacements (86% homology). Two replacements of arginine by lysine and asparagine by serine in positions 15 and 16, respectively, are the most significant. The latter replacement is accompanied by a change in the glycosylation site and might alter its three-dimensional structure. Further studies of the isolate genome are under way.
对1976年至1985年在苏联从野生鸟类中分离出的H13型流感病毒的抗原结构进行了研究。使用针对参考毒株A/海鸥/马里兰/704/77（H13N6）的抗血清来证明这些病毒之间的抗原变异。两种A/H13N6毒株，即A/海鸥/马里兰/704/77和A/大黑背鸥/阿斯特拉罕/227/84，在99 - 215区域的核苷酸序列同源性分别为75%和86%。在A/灰背鸥/阿斯特拉罕/277/84中观察到9个碱基片段的缺失。对这些毒株血凝素在相应区域（氨基酸2 - 40）的预测氨基酸序列进行比较，发现有5个替换（同源性为86%）。分别在第15和16位的精氨酸被赖氨酸替换以及天冬酰胺被丝氨酸替换是最显著的。后一种替换伴随着糖基化位点的改变，可能会改变其三维结构。对分离株基因组的进一步研究正在进行中。
