Wang Zu-Jyun, Kikutani Yuto, Nguyen Lam Thanh, Hiono Takahiro, Matsuno Keita, Okamatsu Masatoshi, Krauss Scott, Webby Richard, Lee Youn-Jeong, Kida Hiroshi, Sakoda Yoshihiro
Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, North 18, West 9, Kita-ku, Sapporo, Hokkaido, 060-0818, Japan.
Training Program for Asian Veterinarians, Japan Veterinary Medical Association, Shin Aoyama Bldg, West 23F, 1-1-1 Minami Aoyama Minato-ku, Tokyo, 107-0062, Japan.
Virus Genes. 2018 Aug;54(4):543-549. doi: 10.1007/s11262-018-1573-0. Epub 2018 May 23.
Among 16 haemagglutinin (HA) subtypes of avian influenza viruses (AIVs), H13 AIVs have rarely been isolated in wild waterfowl. H13 AIVs cause asymptomatic infection and are maintained mainly in gull and tern populations; however, the recorded antigenic information relating to the viruses has been limited. In this study, 2 H13 AIVs, A/duck/Hokkaido/W345/2012 (H13N2) and A/duck/Hokkaido/WZ68/2012 (H13N2), isolated from the same area in the same year in our surveillance, were genetically and antigenically analyzed with 10 representative H13 strains including a prototype strain, A/gull/Maryland/704/1977 (H13N6). The HA genes of H13 AIVs were phylogenetically divided into 3 groups (I, II, and III). A/duck/Hokkaido/W345/2012 (H13N2) was genetically classified into Group III. This virus was distinct from a prototype strain, A/gull/Maryland/704/1977 (H13N6), and the virus, A/duck/Hokkaido/WZ68/2012 (H13N2), both belonging to Group I. Antigenic analysis indicated that the viruses of Group I were antigenically closely related to those of Group II, but distinct from those of Group III, including A/duck/Hokkaido/W345/2012 (H13N2). In summary, our study indicates that H13 AIVs have undergone antigenic diversification in nature.
在禽流感病毒（AIV）的16种血凝素（HA）亚型中，H13 AIV在野生水禽中很少被分离到。H13 AIV引起无症状感染，主要在鸥和燕鸥种群中维持；然而，与该病毒相关的已记录抗原信息有限。在本研究中，从我们监测的同一年同一地区分离出的2株H13 AIV，即A/duck/Hokkaido/W345/2012（H13N2）和A/duck/Hokkaido/WZ68/2012（H13N2），与包括原型株A/gull/Maryland/704/1977（H13N6）在内的10株代表性H13毒株进行了基因和抗原分析。H13 AIV的HA基因在系统发育上分为3组（I、II和III）。A/duck/Hokkaido/W345/2012（H13N2）在基因上被归类为第III组。该病毒与原型株A/gull/Maryland/704/1977（H13N6）以及均属于第I组的A/duck/Hokkaido/WZ68/2012（H13N2）病毒不同。抗原分析表明，第I组病毒与第II组病毒在抗原上密切相关，但与包括A/duck/Hokkaido/W345/2012（H13N2）在内的第III组病毒不同。总之，我们的研究表明H13 AIV在自然界中已经发生了抗原多样化。
