De Maria Carmelo, Burchielli Silvia, Salvadori Claudia, Santoro Vito, Montemurro Francesca, Orsi Gianni, Vozzi Giovanni
Research Center "E. Piaggio," University of Pisa, 56126, Pisa, Italy.
Dipartimento di Ingegneria dell'Informazione, University of Pisa, 56126, Pisa, Italy.
J Biomed Mater Res B Appl Biomater. 2016 Aug;104(6):1220-8. doi: 10.1002/jbm.b.33468. Epub 2015 Jun 19.
The tissue integration and the formation of adhesions in the repair of abdominal wall defects are principally led to the topology and the mechanical properties of implanted prosthesis. In this study we analyzed the influence of the topology of the meshes for abdominal wall repair, made of polypropylene (PP), evaluating its ability to prevent and to minimize the formation of adhesions, and to promote tissue ingrowth. Two series of in vivo studies were performed. In the first, two types of PP meshes, a lightweight macroporous mesh (LWM) and a heavyweight microporous mesh (HWM) were compared with determine the optimal porosity for tissue integration. In the second, a composite mesh, Clear Mesh Composite (CMC), made of a LWM sewn on a PP planar smooth film, was compared with a PP planar film, to demonstrate how two different topologies of same material are able to induce different tissue integration with the abdominal wall and different adhesion with internal organs. In both studies, the prostheses were implanted in Wistar rats and histological analysis and mechanical characterization of tissue coupled with the implants were performed. LWM showed better host tissue ingrowth in comparison to HWM. CMC prosthesis showed no adhesions to the viscera and no strong foreign body reaction, moreover its elasticity and anisotropy index were more similar to that of natural tissue. These results demonstrated that the surface morphology of PP surgical meshes allowed to modulate their repair ability. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1220-1228, 2016.
腹壁缺损修复过程中的组织整合及粘连形成主要取决于植入假体的拓扑结构和力学性能。在本研究中，我们分析了由聚丙烯（PP）制成的用于腹壁修复的网片拓扑结构的影响，评估其预防和最小化粘连形成以及促进组织向内生长的能力。进行了两组体内研究。第一组，比较了两种类型的PP网片，即轻质大孔网片（LWM）和重质微孔网片（HWM），以确定组织整合的最佳孔隙率。第二组，将由缝在PP平面光滑薄膜上的LWM制成的复合网片Clear Mesh Composite（CMC）与PP平面薄膜进行比较，以证明相同材料的两种不同拓扑结构如何能够诱导与腹壁不同的组织整合以及与内部器官不同的粘连情况。在两项研究中，均将假体植入Wistar大鼠体内，并对与植入物相连的组织进行组织学分析和力学特性表征。与HWM相比，LWM显示出更好的宿主组织向内生长情况。CMC假体未显示与内脏的粘连且无强烈的异物反应，此外其弹性和各向异性指数更类似于天然组织。这些结果表明，PP手术网片的表面形态能够调节其修复能力。© 2015威利期刊公司。《生物医学材料研究杂志》B部分：应用生物材料，104B: 1220 - 1228，2016年。
