Lu S, Hu W, Zhang Z, Ji Z, Zhang T
Department of General Surgery, Affiliated ZhongDa Hospital (Jiang Bei), Southeast University, No. 211 Jianmin Road, Nanjing, 210009, Jiangsu, China.
Department of General Surgery, Institute for Minimally Invasive Surgery, Affiliated ZhongDa Hospital, Medical School, Southeast University, No. 87 Dingjiaqiao Road, Nanjing, 210009, Jiangsu, China.
Hernia. 2018 Dec;22(6):1051-1060. doi: 10.1007/s10029-018-1782-4. Epub 2018 May 18.
This study evaluated the manufacturing method and anti-adhesion properties of a new composite mesh in the rat model, which was made from sirolimus (SRL) grafts on a poly(L-lactic acid) (PLLA)-modified polypropylene (PP) hernia mesh.
PLLA was first grafted onto argon-plasma-treated native PP mesh through catalysis of stannous chloride. SRL was grafted onto the surface of PP-PLLA meshes using catalysis of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 4-dimethylaminopyridine (DMAP) in a CHCl solvent. Sprague-Dawley female rats received either SRL-coated meshes, PP-PLLA meshes, or native PP meshes to repair abdominal wall defects. At different intervals, rats were euthanized by a lethal dose of chloral hydrate and adhesion area and tenacity were evaluated. Sections of the mesh with adjacent tissues were assessed histologically.
Attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy indicated the existence of a C=O group absorption peak (1724.1 cm), and scanning electron microscope morphological analysis indicated that the surface of the PP mesh was covered with SRL. Compared to the native PP meshes and PP-PLLA meshes, SRL-coated meshes demonstrated the greatest ability to decrease the formation of adhesions (P < 0.05) and inflammation.
The SRL-coated composite mesh showed minimal formation of intra-abdominal adhesions in a rat model of abdominal wall defect repair.
本研究在大鼠模型中评估了一种新型复合补片的制造方法和抗粘连性能，该补片由聚（L-乳酸）（PLLA）改性聚丙烯（PP）疝修补补片上的西罗莫司（SRL）移植物制成。
首先通过氯化亚锡催化将PLLA接枝到经氩等离子体处理的天然PP补片上。在CHCl溶剂中，使用1-（3-二甲基氨基丙基）-3-乙基碳二亚胺盐酸盐（EDC）和4-二甲基氨基吡啶（DMAP）催化，将SRL接枝到PP-PLLA补片表面。将雌性Sprague-Dawley大鼠分别植入SRL包被的补片、PP-PLLA补片或天然PP补片以修复腹壁缺损。在不同时间间隔，通过致死剂量的水合氯醛对大鼠实施安乐死，并评估粘连面积和粘连强度。对带有相邻组织的补片切片进行组织学评估。
衰减全反射傅里叶变换红外光谱（ATR-FTIR）表明存在C=O基团吸收峰（1724.1 cm），扫描电子显微镜形态分析表明PP补片表面覆盖有SRL。与天然PP补片和PP-PLLA补片相比，SRL包被的补片在减少粘连形成（P < 0.05）和炎症方面表现出最强的能力。
在腹壁缺损修复的大鼠模型中，SRL包被的复合补片显示出腹腔内粘连形成最少。
