Ganesan Kesavan, Nirmal R Madhavan, Nassar M Mohamed, Veeravarmal V, Amsaveni R, Kumar Arul
Department of Oral Pathology, Madha Dental College and Hospital, Chennai, India.
Department of Oral Pathology, Rajah Muthiah Dental College and Hospital, Chidambaram, Tamil Nadu, India.
J Oral Maxillofac Pathol. 2015 Jan-Apr;19(1):42-6. doi: 10.4103/0973-029X.157200.
Oral squamous cell carcinoma (OSCC) is the most common malignancy of the oral mucosa. Stromal myofibroblasts play an important role in tumor invasion and metastasis, due to its ability to modify the extracellular matrix. The purpose of this study was to evaluate and compare the presence of myofibroblasts in normal mucosa, early invasive carcinoma and different grades of OSCC.
The study included the archival tissues of 18 OSCC of well, moderate and poorly differentiated grades, three early invasive carcinomas and five normal mucosa. Myofibroblasts were identified by immunohistochemical detection of h1 calponin.
The percentage and intensity of h1 calponin were examined and positive immunostaining was observed in the myofibroblasts of all SCCs and early invasive carcinomas; however, these cells did not stain in the normal epithelium specimens. The presence of myofibroblasts was significantly higher in invasive pattern of OSCCs compared to normal mucosa cases (P < 0.070). A significant difference was not observed between the different grades of OSCC (P ≤ 0.812).
These findings show the presence of myofibroblasts in OSCC but not in normal mucosa, suggesting that the genetically altered epithelium (carcinomatous epithelium) may have an inductive effect on the adjacent stroma to produce myofibroblasts. Also transdifferentiation of myofibroblasts is induced somewhere in the invasive stage of SCC irrespective of the epithelial cell differentiation.
口腔鳞状细胞癌（OSCC）是口腔黏膜最常见的恶性肿瘤。基质肌成纤维细胞由于能够修饰细胞外基质，在肿瘤侵袭和转移中起重要作用。本研究的目的是评估和比较正常黏膜、早期浸润癌及不同分级的OSCC中肌成纤维细胞的存在情况。
本研究纳入了18例高分化、中分化和低分化OSCC的存档组织、3例早期浸润癌和5例正常黏膜组织。通过免疫组织化学检测h1钙调蛋白来鉴定肌成纤维细胞。
检测h1钙调蛋白的百分比和强度，在所有SCC和早期浸润癌的肌成纤维细胞中均观察到阳性免疫染色；然而，这些细胞在正常上皮标本中未染色。与正常黏膜病例相比，OSCC浸润型中肌成纤维细胞的存在显著更高（P < 0.070）。不同分级的OSCC之间未观察到显著差异（P ≤ 0.812）。
这些发现表明OSCC中存在肌成纤维细胞而正常黏膜中不存在，提示基因改变的上皮（癌上皮）可能对邻近基质有诱导作用以产生肌成纤维细胞。此外，无论上皮细胞分化情况如何，在SCC浸润阶段的某个部位都会诱导肌成纤维细胞的转分化。
