Berean Kyle J, Adetutu Eric M, Zhen Ou Jian, Nour Majid, Nguyen Emily P, Paull David, Mcleod Jess, Ramanathan Rajesh, Bansal Vipul, Latham Kay, Bishop-Hurley Greg J, McSweeney Chris, Ball Andrew S, Kalantar-Zadeh Kourosh
School of Electrical and Computer Engineering, RMIT University, Melbourne, Australia, 3000.
School of Applied Science, RMIT University, Bundoora, Australia, 3083.
Sci Rep. 2015 Jun 22;5:11515. doi: 10.1038/srep11515.
Unique in vivo tests were conducted through the use of a fistulated ruminant, providing an ideal environment with a diverse and vibrant microbial community. Utilizing such a procedure can be especially invaluable for investigating the performance of antimicrobial materials related to human and animal related infections. In this pilot study, it is shown that the rumen of a fistulated animal provides an excellent live laboratory for assessing the properties of antimicrobial materials. We investigate microbial colonization onto model nanocomposites based on silver (Ag) nanoparticles at different concentrations into polydimethylsiloxane (PDMS). With implantable devices posing a major risk for hospital-acquired infections, the present study provides a viable solution to understand microbial colonization with the potential to reduce the incidence of infection through the introduction of Ag nanoparticles at the optimum concentrations. In vitro measurements were also conducted to show the validity of the approach. An optimal loading of 0.25 wt% Ag is found to show the greatest antimicrobial activity and observed through the in vivo tests to reduce the microbial diversity colonizing the surface.
通过使用造瘘反刍动物进行了独特的体内试验，提供了一个具有多样且活跃的微生物群落的理想环境。利用这样的程序对于研究与人类和动物相关感染有关的抗菌材料的性能可能特别有价值。在这项初步研究中，结果表明造瘘动物的瘤胃为评估抗菌材料的性能提供了一个出色的活体实验室。我们研究了基于不同浓度银（Ag）纳米颗粒与聚二甲基硅氧烷（PDMS）制成的模型纳米复合材料上的微生物定植情况。由于可植入设备对医院获得性感染构成重大风险，本研究提供了一个可行的解决方案，以了解微生物定植情况，并有可能通过引入最佳浓度的银纳米颗粒来降低感染发生率。还进行了体外测量以证明该方法的有效性。发现0.25 wt% Ag的最佳负载量显示出最大的抗菌活性，并通过体内试验观察到可减少定植在表面的微生物多样性。
